中文摘要：

目的：建立复方阿胶浆中阿胶的专属性检测方法。方法：采用胰蛋白酶对复方阿胶浆中阿胶成分进行酶解,利用超高效液相色谱-三重四极杆质谱（RRLC-QQQ/MS）对阿胶的专属性特征分子离子峰进行检测。采用Agilent SB-C18（2.1 mm×100 mm,1.8μm）色谱柱,以0.1%甲酸水溶液为流动相A,以乙腈为流动相B,梯度洗脱（0～25 min,95%A→80%A,5%B→20%B;25～40 min,80%A→50%A,20%B→50%B）。流速0.3 mL·min^-1,柱温40℃,进样量5μL;选择阿胶特征分子离子峰m/z 539.8（双电荷）→612.4和m/z 539.8（双电荷）→923.8作为检测离子对,离子化模式为ESI＋,进行多反应监测。结果：3批市售样品中均可检出阿胶的特征分子离子峰,即同时检出m/z 539.8（双电荷）→612.4和m/z 539.8（双电荷）→923.8离子对。结论：所建立的方法经方法学验证,阴性样品及其他胶类如黄明胶、龟甲胶和鹿角胶等对复方阿胶浆样品测定无干扰,样品检出浓度为含5%阿胶的复方阿胶浆样品。方法专属性强,可用于复方阿胶浆中阿胶的检测。

英文摘要：

Objective： To establish an analytical method for identification of donkey-hide gelatin in Fufang Ejiao Jiang. Methods： Identification of donkey-hide gelatin in Fufang Ejiao Jiang was established by rapid resolution liquid chromatography（ RRLC） coupled to triple quadruple mass spectrometry（ QQQ-MS）. Chromatographic separation was carried out on an Agilent Zorbax SB-C18 reversed phase analytical column（ 100 mm × 2. 1 mm,1. 8 μm particle size） at a column temperature of 40℃. The injected sample volume was 5 μL. The mobile phase consisted of0. 1% formic acid in water（ eluent A） and acetonitrile（ eluent B）. The gradient elution was performed as follows：0-25 min eluent A 95% → 80%,elutent B 5% → 20%; 25-40 min,eluent B 20% → 50%. A flow rate of0. 3 mL·min^-1was employed for elution. Collagen marker peptides were detected by mass spectrometry in the positive electrospray ionization with multiple reaction monitoring（ MRM）. Results： The characteristic molecular peaks of donkey-hide gelatin m / z 539. 8→612. 4 and m / z 539. 8→923. 8 were detected in all three batches of commercial samples. Conclusion： The methodological verification showed that the donkey-hide gelatin was not interfered with the negative gelatins and other gelatins such as bovine-hide gelatin,deer-horn glue and tortoise shell gelatin. The detection limit of the new method extended to 5%. The present method was specific,precise and reliable,and was suitable for identification of donkey-hide gelatin in Fufang Ejiao Jiang.