中文摘要：

在碱性介质中，6-巯基嘌呤（6-MP）与过氧化氢反应可在405nm波长处产生微弱的荧光，微量铜（Ⅱ）的存在能大大增敏其荧光强度。据此，建立了一种高灵敏测定6-MP的荧光光度分析新方法。在优化的条件下，体系的荧光强度与6-MP的浓度在2．1×10^-8～1．7×10^-5mol·L^-1范围内呈线性关系，检出限为7．1×10^-9mol·L^-1。方法用于片剂中6-MP含量的测定，其结果与药典方法对照，令人满意。

英文摘要：

In basic media, 6-mercaptopurine reacts with hydrogen peroxide to form a new fluorescent compound,which possesses a weak emission wavelength of 405 nm. Its fluorescence intensity can be enhanced remarkably when trace Cu^2＋ exists as catalyst. Based on this, a novel spectrofluorimetric method with high sensitivity was developed for the determination of 6-mercaptopurine. Under the optimal conditions,the calibration graphs are linear over the range of 2.1 ×10^-8～1.7 ×10^-5 mol·L^-1 with a detection limit of 7.1×10^-9 mol·L^-1. The proposed method was successfully applied to determine 6-mercaptopurine in pharmaceutical preparations, the results obtained by pharmacopoeia method.