中文摘要：

目的利用响应面分析法对桦褐孔菌发酵培养基进行优化,提高多糖产量。方法应用Plackett-Burman设计和响应面法,对桦褐孔菌发酵培养基成分进行优化。用Design-Expert软件对实验数据进行多元回归分析,并建立3种主要因素（淀粉、酵母粉和磷酸二氢钠）与多糖产量之间的函数关系。用最终优化的配方进行3次验证实验。结果通过快速＂登高法＂对3个显著因素进行寻优,培养基中3因素最佳浓度为：淀粉7.5 g/L,酵母粉1.71 g/L,磷酸二氢钠1.35 g/L,胞外多糖产量最高可达9.12 g/L。3次验证实验所测得的多糖产量分别为9.08、8.96和9.12 g/L,平均值为9.05 g/L,与预测结果基本一致。结论优化的桦褐孔菌发酵培养基可显著提高胞外多糖产量。

英文摘要：

Objective To optimize the medium for fermentation of Fuscoporia obliqua by response surface method and increase the yield of polysaccharide.Methods The component of medium for fermentation of Fuscoporia obliqua was optimized by Plackett-Burman design and response surface method.Polynomial regression analysis was performed on the experimental data by using Design-Expert software,based on which the functional relationship between polysaccharide yield and three main factors（starch,yeast extract and sodium dihydrogen phosphate） were explored.The finally optimized formula of medium was subjected to three verification test.Results The optimal concentrations of starch,yeast extract and sodium dihydrogen phosphate were 7.5,1.71 and 1.35 g / L respectively,and the extracellular polysaccharide yield reached 9.12 g / L at most.The polysaccharide yields obtained by three verification tests were 9.08,8.96 and 9.12 g / L respectively,with a mean of 9.05 g / L,which was basically consistent with that expected.Conclusion By using the optimized medium,the extracellular polysaccharide yield increased significantly.