中文摘要：

目的探讨氧还蛋白过氧化物2基因在表皮干细胞向汗腺细胞分化过程中对表型改变的影响。方法分离培养健康包皮获取表皮干细胞，分离培养健康成人皮肤获取汗腺细胞，以免疫荧光细胞化学染色鉴定。慢病毒载体介导的氧还蛋白过氧化物2基因分为过表达组、敲低表皮干细胞组及空白对照组，RT．PCR和WesternBlot技术分别在基因和蛋白水平检测3组的氧还蛋白过氧化物2表达。每组分别通过Transwell板与汗腺细胞共培养，流式细胞术检测共培养前、后表皮干细胞CEA和B1整合素的表达情况。结果表皮干细胞及汗腺细胞符合其相应特异性抗原。共培养前表皮干细胞B1整合素高表达（98．69±0．67）％，CEA几乎不表达（6．20±3．15）％；共培养后B1整合素表达程度：敲低组（19．30±0．53）％〈空白对照组（65．77±2．32）％〈过表达组（92．63±10．97）％；共培养后CEA表达程度：敲低组（95．43±2．36）％〉空白对照组（51．20±0．79）％〉过表达组（45．91±0．93）％。以上结果组间比较差异有统计学意义（P〈0．05）。结论氧还蛋白过氧化物2基因可抑制表皮干细胞向汗腺细胞分化的表型改变，并可提高保留其本身特异性抗原表达水平的能力。

英文摘要：

Objective To investigate the effects of PRX-2 gene on phenotype changes in epidermal stem cells differentiating into sweat gland cells. Methods Epidermal stem ceils and sweat gland cells separated and cultured from healthy foreskin and adult full-thick skin respectively, were identified by immunofluorescence staining. Lentiviral vector-mediated overexpression and knockdown of PRX-2 gene in epidermal stem cells were performed respectively, with empty vector-mediated epidermal stem cells as a control group. Overexpression,hlank control and knowdown group＇s PRX-2 expressions in gene and protein levels were detected using RT-PCR and Western blot technology. The ESCs of each group were co-cultured with sweat gland cells through transwell plate, and the expressions of CEA and β1 integrin in epidermal stem cells were determined by flow cytometry before and after co-culturing. Results Epidermal stem cells and sweat gland cells were in line with their respective specific antigens. Before co-cultured, epidermal stem cells highly expressed β1 integrin （98.69 ± 0.67 ）% , hardly expressed CEA （6.20 ± 3.15 ） %. After co-cultured, β1 integrin expression levels were showed as knockdown group （19.30 ± 0. 53 ）% 〈 blank control group （65.77 ± 2.32） % 〈 overexpress group （92.63 ± 10. 97 ） % , and CEA expression levels as knockdown （ 95.43 ± 2.36） % 〉 blank control group （ 51.20 ±0.79） % 〉 overexpress group （45.91±0.93）%. There had significant differences between those of each two groups. Conclusions PRX-2 gene can inhibit the phenotypic change of Epidermal Stem Cells differentiating into Sweat Gland Ceils and improve the ability to maintain their own specific antigens.