中文摘要：

文中研究在粒状铁化学还原三氯乙烯渗透反应格栅和生物降解苯和甲苯渗透反应格栅的联合格栅技术中,下游生物降解格栅中铁还原环境下微生物对苯和甲苯的生物降解。通过模拟粒状铁渗透反应格栅下游铁还原环境,主要研究微生物以苯、甲苯作为碳源时,对碳源的专一性。通过批实验发现,对于分别用苯和甲苯培养驯化的微生物,互换碳源后,微生物对2 000.00μg/L苯和甲苯的生物降解半衰期分别由之前的1.0和1.5 d减少到0.7和1.0 d,互换碳源后苯和甲苯的去除率分别增加38%和32%。在粒状铁渗透反应格栅下游生物降解渗透反应格栅中,微生物在铁还原环境下以苯、甲苯作为碳源时对碳源没有专一性,苯和甲苯可以同时被生物降解去除。

英文摘要：

This study simulated benzene and toluene biodegradation under an iron reducing environment in a downstream biodegradation permeable reactive barrier（Bio-PRB） of a composite permeable reactive barrier which includes an upstream zero-valent permeable reactive barrier（ZVI PRB） that reduces trichloroethene.Microbial specificity to a carbon source was mainly evaluated by batch experiments where benzene and toluene were used as the carbon sources.After exchanging the carbon sources,the biodegradation half-life of 2000.00 μg/L of benzene and toluene decreased from 1.16 days to 0.71 days and from 1.45 days to 0.98 days,respectively.In addition,the removal increased by 38.80% for benzene and by 32.41% for toluene compared to the microbe degradation without the exchange of the carbon sources.Thus,for benzene and toluene degradation in a downstream bio-PRB behind an upstream ZVI PRB,microbes have no specificity to a carbon source;microbes can degrade benzene and toluene together.