中文摘要：

为了探索土壤酸化对红耳龟Trachemys scripta elegans和中华条颈龟Mauremys sinensis卵孵化和非特异性免疫功能的影响,实验设置孵化温度（30±0.5）℃,孵化基质pH为4、5、6、7条件下,对比研究两种龟卵孵化率、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量的变化。结果显示,两种龟卵孵化率均随孵化基质pH的降低而降低,pH7、6、5同种龟不同处理组间和同种处理不同种间孵化率均差异不显著（P〉0.05）,pH4可使龟卵致死;pH5、6组肝脏和肌肉中SOD和CAT活性均显著低于对照组pH7（P〈0.05）,且随着pH的降低,SOD、CAT活性均呈下降趋势,MDA含量相反呈上升趋势;不同物种间肝脏和肌肉SOD、CAT活性均是红耳龟大于中华条颈龟,MDA含量则小于中华条颈龟（P〈0.05）,表明胚胎期应对酸胁迫,中华条颈龟的钙质卵壳略占优势,红耳龟主要通过增强非特异性免疫机能提高抗氧化能力,在生理调节方面表现出较强的机体免疫能力和防御能力。

英文摘要：

This study was designed to evaluate the toxic effects of different hatching medium acidification（pH=4,5,6,7） on embryonic period of Trachemys scripta elegans and Mauremys sinensis.The effects on hatching rates and nonspecific immunity mechanism of the two species were contrasted by simulating soil acidification environment with different pH.Results indicated that hatching rates of the two species decreased as pH of hatching medium decreased.When pH approximately approaching to 4,the hatching rates of two species were both null,and there were no significant difference between the same species under different treatments or the same treatment of different treatments（P0.05）.Superoxide dismutase（SOD） and catalase（CAT） activity in liver and intestine of control group（pH=7） of the two species were significantly higher than that of experimental groups（pH=5,pH=6）（P0.05） and declined with the decrease of pH,while malondialdehyde（MDA） contents presented an opposite tendency.The activity of SOD and CAT in liver and intestine of T.s.elegans were higher than that of M.sinensis when pH equaled,however MDA contents were not.Therefore,these results indicated that dealing with acid stress during embryonic period,M.sinensis showed slightly predominant relying on calcareous egg shell,while T.s.elegans improves the anti-oxidant ability mainly by enhancing nonspecific immunity function and thus showing stronger defense ability and immunologic competence in physiological adjustment.