中文摘要：

以铁炮百合（Lilium longiforum）‘白天堂’的组培苗叶片为试材，通过RACE方法得到了一个热激转录因子（heat shock transcription factor，HSF）基因LlHSF1的cDNA全长序列。该序列全长1 068 bp，推断其含有一个780 bp的开放阅读框（ORF），编码260个氨基酸，推导的蛋白质分子量为30.123kD。对推定的氨基酸序列与其它已知物种HSF进行比对发现，该基因有热激转录因子所具备的典型结构域和调控元件，据此推断，克隆到的基因是一个新的热激转录因子成员，命名为LlHSF1。荧光定量PCR分析结果表明：常温下该基因在百合根、鳞茎、叶中都有表达，但在叶片中表达量较高；实时荧光定量PCR分析结果表明，42℃处理1-12 h，叶中该基因表达量增加。

英文摘要：

A full length cDNA named LlHSF1,belonging to Heat shock transcription factor（HSF）,was cloned from Lilium longiforum‘White Heaven’by RT-PCR and RACE（rapid amplification of cDNA ends）. The gene consisted of 1 068 bp and open reading frame encompassed 780 bp encoding a polypeptide of 260 amino acids with calculated protein molecular mass of 30.123 kD. Cluster analysis showed that this gene was highly homologous to known HSF from other organisms. RT-PCR analysis showed that expression of LlHSF1 could be detected in leaf,root and bulb at 22 ℃,and it was expressed highly in the leaf.Theresults with SYBR GREEN real-time quantitative PCR showed that the gene expression level increased in leaves during 1–12 hours at 42 ℃.