中文摘要：

目的构建pEGFP—Ngb荧光表达载体并研究其在缺氧条件下SY5Y细胞中的功能。方法小鼠NgbeDNA被亚克隆进pEGFP—N1载体，重组载体转染SY5Y细胞，通过双苯并咪唑染料（Hoechest）和碘化丙啶（PI）染色观测细胞在低氧条件的抗缺氧作用。结果成功构建pEGFP—Ngb荧光表达载体，表达Ngb的SY5Y细胞在低氧条件下的存活率增加。结论Ngb在低氧条件下对SY5Y细胞存活可能具有重要作用。

英文摘要：

Objective： To construct pEGFP- Ngb expression vector and to investigate its function in SY5Y cells under hypoxia condition. Methods The Ngb eDNA of mice was subcloned into pEGFP - N1 vector and the recombinant vector：was transfected into SY5Y cells to observe its antihypoxic function by Hoechest and PI dyeing. Results： The pEGFP- Ngb, oxpression vector was successfully constructed. The survival rate of SY5Y cells which expressed Ngb was increased under hypoxia condition. Conclusion： Ngb may play an important role in cell survive under hypoxla condition.