为了解松突圆蚧Hemiberlesia pitysophila Takagi对海拔的热适应性,通过极端温度暴露试验和过冷却点测定,比较了不同海拔梯度(80~725m)上冬季松突圆蚧1龄若虫、2龄性分化前若虫和雌成虫的热耐受性。结果表明:(1)不同海拔松突圆蚧的热耐受性具有显著差异,主要表现为海拔391m区域2龄性分化前若虫和雌成虫的耐寒性,以及雌成虫的耐热性均显著较强 除2龄性分化前若虫的耐寒性外,该虫的热耐受性未表现出随海拔升高而显著增强的趋势。这一结果不支持气候变化假说,但说明海拔是限制松突圆蚧种群扩张和地理分布的重要因素。(2)海拔梯度上,1龄若虫和2龄性分化前若虫中耐寒性与耐热性的相关性均不显著,但在雌成虫中呈显著正相关,说明海拔梯度上松突圆蚧耐寒性和耐热性的关系与其发育阶段有关。(3)海拔梯度上,雌成虫的过冷却点与其致死中低温的相关性未表现出显著水平。本文结果有助于深入理解松突圆蚧的热适应性和地理分布特征。
In order to explore the thermal adaptability to elevation in the pine armored scale (PAS),Hemiberlesia pitysophila Takagi,an invasive forest pest originated in Japan which had been severely attacking various ever green pines in southern China in the recent two decades,the cold and heat tolerances of 1st instar nymphs,2nd instar nymphs before sexual differentiation and adult females of the winter field populations of PAS collected from five plots at different elevation (80,251,391,510 and 725 m,respectively) were compared,based on the survival experiments exposed to extreme temperature conditions in Quanzhou,Fujian in February,2009 and the measures of supercooling point (SCP). The results showed that (1) thermal tolerances of PAS at different elevation had significant differences,in which adult females and 2nd instar nymphs all displayed higher cold tolerance at the elevation of 391 m,and an increased heat tolerance in adult females at the same elevation was observed too. Cold tolerance seemed to increase with increasing of elevation in the 2nd instar nymphs,but not in other developmental phases. This finding though provides an opponent case of climate variation hypothesis,it suggests that elevation might be an important limit to population expansion and geographic distribution of PAS. (2) Pearson's correlation between cold and heat tolerance in adult females was statistically remarkable and positive along an altitudinal gradient,but no correlations existed in other developmental phases,suggesting that the developmental phase of PAS might affect this correlation. (3) A separate analysis showed no marked Pearson's correlation between SCP and median lethal low temperature of adult females in PAS exposed for 4 h along elevation gradient. These results provide a helpful foundation to profoundly understand thermal adaptability and geographic distribution in PAS.