中文摘要：

目的：探讨萎缩肌纤维中Nogo-A蛋白的表达对诊断肌萎缩侧索硬化（ALS）的意义。方法：收集经临床、病理或基因检测明确诊断的患者40例,其中ALS 12例,非ALS神经源性损害8例,肌源性损害20例,对所有40例肌肉冰冻组织标本行Nogo-A免疫组织化学染色。结果：无论是在神经源性损害还是肌源性损害的疾病中出现的萎缩肌纤维均有Nogo-A蛋白表达。利用NADH-TR和ATPase染色证实表达Nogo-A的萎缩肌纤维以Ⅰ型肌纤维为主。结论：Nogo-A并不仅仅存在于ALS患者的肌肉中,它也可以在其他疾病所致的萎缩肌肉中表达,因而Nogo-A表达与否并不能作为诊断ALS的标准。

英文摘要：

Objective：To investigate significance of Nogo-A in atrophic muscle fibers in diagnosis of amyotrophic lateral sclerosis（ALS）.Methods： Forty cases which were diagnosed definitely by clinical,pathological or DNA analysis were included.All of the cases underwent muscle biopsies in order to carry out Nogo-A immunostaining.Results： Nogo-A expression was detected in the atrophic muscle fibers but in either neurogenic disease or myogenic disease,the atrophic muscle fibers demonstrated expression of Nogo-A.As compared with the stainings of NADH-TR and ATPase,it was showed that Nogo-A positive fibers were mainly type Ⅰ fibers.Conclusion： Our results show that the presence of Nogo-A in diseased human muscle biopsies is not limited to ALS,therefore it cannot be the standard for ALS diagnosis.