中文摘要：

目的：利用免疫组织化学的方法探讨小G蛋白Ran在胃癌中的表达及临床意义。方法：利用免疫组织化学染色法研究74例胃癌组织标本（其中高分化25例,中分化24例,低分化25例）及其毗邻正常组织中Ran的表达情况,并分析该蛋白表达水平与临床病理参数之间的关联。结果：（1）Ran在胃癌组织中的染色强度明显高于正常组织。（2）在癌组织中Ran表达于胞核和胞浆,其中又以胞核为主,在正常组织中Ran主要表达于胞浆。（3）Ran的表达与患者年龄、性别无相关性（0.464、0.912）,与肿瘤分化、TNM分期和转移与否有显著相关性（0.001、〈0.001、〈0.001）。结论：与正常组织相比,Ran在胃癌组织中的表达显著增高,并且与肿瘤分化和病理分期存在显著正相关,其可能作为胃癌新的分子标志物,在肿瘤的发生发展中发挥重要作用。

英文摘要：

Objective： To investigate the expression of the small G protein Ran in gastric cancer and its clinical significance.Methods： Immunohistochemistry was used to detect the expression of Ran in 74 cases of gastric tumor tissues,among which there were 25 well-differentiated cases,24 moderately-differentiated cases,and 25 poorly-differentiated cases,and their adjacent normal tissues.Analyze the relevance between the expression and the clinic pathological parameters.Results：（1） Ran was expressed greater in gastric cancer tissues than in normal gastric mucosa by immunohistochemical study.（2） In gastric cancer tissues,Ran was expressed in both nucleus and cytoplasm,but mainly in nucleus.However,in normal tissues,its expression was localized in cytoplasm.（3） Expression of Ran had no correlation with the patients＇ age and gender（p=0.464,0.912）,but was positively correlated with tumor differentiation（P=0.001）,invasion（P〈0.001）,and pathological stage（P〈0.001）.Conclusion： Ran expression is up-regulated in gastric cancer and positively correlated with tumor differentiation and pathological stage.It could be taken as a new potential biomarker of gastric cancer,and may play an important role in the development of gastric cancer.Ranr;Gastric cancer;Small G Protein