中文摘要：

目的 制备并鉴定蓝氏贾第鞭毛虫（简称贾第虫, Giardia lamblia）单克隆抗体（mAb）。方法 以贾第虫滋养体抗原免疫2只8周龄雌性BALB/c小鼠, 利用细胞融合技术建立抗贾第虫抗原杂交瘤细胞株并制备mAb。制备贾第虫滋养体可溶性抗原（STA）和排泄-分泌抗原（ESP）, 采用免疫球蛋白及亚型检测试剂盒鉴定mAb的类型及亚型。蛋白质印迹（Western blotting）鉴定mAb对贾第虫滋养体STA和ESP的识别。采用ELISA检测mAb与贾第虫滋养体抗原反应性, 并检测与其他寄生虫抗原的交叉反应。结果 获得12株分泌贾第虫单克隆抗体细胞株（BB3、CE5、CC10、EG4、GC7、CC1、EF6、DB8、BG10、GH7、HC7和EB2）, 均为IgG1亚型。其中EB2能识别贾第虫滋养体STA和ESP中相对分子质量分别为175 000和191 000的蛋白条带, 与大肠埃希菌（Escherichia coli）、猪蛔虫（Ascaris suum）、人芽囊原虫（Blastocystis hominis）、日本血吸虫（Schistosoma japonicum）虫卵、日本血吸虫成虫和卫氏并殖吸虫（Paragonimus westermani）成虫抗原等均无交叉反应。结论 制备的贾第虫特异性mAb为IgG1亚型。

英文摘要：

Objective To prepare and identify the monoclonal antibodies （mAb） against Giardia lamblia.Methods Two BALB/c mice of 8 weeks were immunized with antigens extracted from G. lamblia trophozoites, based on which mAb was prepared with traditional hybridoma technology. mAb isotyping was performed using enzyme-linked immunosorbent assay （ELISA） with coatings by soluble trophozoites antigen （STA） and excretory-secretory protein （ESP） prepared from G. lamblia trophozoites. mAb recognition of STA and ESP was identified by Western blotting. ELISA was performed to detect mAb reactions with G. lamblia trophozoite antigen as well as its cross-reactions with antigens from other parasite species. Results Twelve mAb-secreting hybridoma cell lines were obtained, comprising BB3, CE5, CC10, EG4, GC7, CC1, EF6, DB8, BG10, GH7, HC7 and EB2, all producing the IgG1-subtype mAb. The EB2 mAb specifically recognized proteins with Mr of 175 000 and 191 000 from STA and ESP, respectively, with no cross-reactions to the antigen extracts from Escherichia coli, Ascaris suum, Blastocystis hominis, eggs and adult worms of Schistosoma japonicum, and adult worms of Paragonimus westermani. Conclusion The specific IgG1-type mAb for G. lamblia trophozoites has been prepared.