中文摘要：

目的通过对吗啡依赖大鼠视皮层γ-氨基丁酸（GABA）含量及GAD与GABA-T活力的测定，研究吗啡成瘾过程中视皮层γ氨基丁酸的代谢变化。方法30只SD大鼠分为6组，末次给药前10min，后10min，后3h处死组及相应对照组，每组5只。纸电泳法检测各组大鼠视皮层中GABA含量，分光光度法检测GAD与GABA-T的活力。结果吗啡末次给药前处死组大鼠GABAA含量（0．09±0．03）μmol／mg与对照组（0．17±0．05）μmol／mg比较，差异有统计学意义。末次给药后处死组GABA含量回复至对照组水平。结论吗啡依赖形成时视皮层处于去抑制状态。

英文摘要：

Objective To investigate the changes of γ-aminobutyfie aeid（GABA） content and glutamie acid deearboxylase （GAD）, γ-aminobutyrie acid transaminase （GABA-T） activity in visual cortex of morphine-dependent rats. Methods Paper eleetrophoresis was employed to detect GABA content in visual cortex and speetrophotometrie method was used to investigate the activity of GAD and GABA-T in each experimental group. Results GABA content in visual cortex of rats decapitated before the last morphine injection was significantly decreased （0.09± 0.03）μmol/mg compared with saline control （0.17± 0.05）μmol/mg. GABA content of rats decapitated after the last morphine injection restored to normal level. Conclusion Visual cortex is in disinhibition with chronic morphine exposure.