欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
该工作以富含大量胸腺嘧啶（Thymine，T）核酸单链为识别分子，SYBR Green I （SG）为荧光基团，建立了一种简单、灵敏的荧光增强法检测Hg2＋。由于T-Hg2＋-T键的形成，富T单链自我折叠或者两两配对形成双链DNA结构，当溶液中的SG嵌入DNA双链中时，SG荧光强度显著增强。实验结果表明，SG荧光强度随着Hg2＋浓度的增加而增加。在最优实验条件下，SG的荧光强度与Hg2＋的浓度在4．000×10-7~2．000×10-6 mol/L范围内呈线性关系，检出限为3．900×10-8 mol/L。该方法在含5．0％湘江水实际样品中获得的回收率为98．72％~104．5％，因此该传感器可用于实际湘江水样品中Hg2＋的测量。
英文摘要：
Mercury is one of the most toxic heavy metals that present in environment. Therefore, a sensitive and se-lective sensing system for mercury detection is highly demanded. This paper described a fluorescent sensor using a label free Hg2＋specific DNA probe（43mer-T18Stem） and an intercalation dye SYBR Green I（SG）. As is known to all, Hg2＋can specifically interact with thymine base to form strong and stable thymine-Hg2＋-thymine （T-Hg2＋-T） com-plexes. This specific T-Hg2＋-T formation affected the hybridization of the Hg2＋specific probe and the intercalation of SG. The proposed sensor showed a linear response in the range of 4.000×10-7~2.000×10-6 mol/L of Hg2＋with a detec-tion limit of 3.900×10-8 mol/L. The sensor revealed good recovery rates from 98.72%to 102.8%, indicating that the sensing system can be utilized for the determination of Hg2＋in real samples.
同期刊论文项目
同项目期刊论文
Cleaved DNAzyme substrate induced enzymatic cascade for the exponential amplifi ed analysis of L-his
Enzymatic cascade based fluorescent DNAzyme machines for the ultrasensitive detection of Cu(II) ions
Detection of double stranded DNA and its damage by liquiritigenin with copper (II) on multi-walled c
Estimation of Surface Tension of Organic Compounds Using Quantitative Structure Property Relationshi
An electrochemical immunosensor based on gold nanoparticle tags for picomolar detection of c-Myc onc
Myristic acid/polyaniline composites as form stable phase change materials for thermal energy storag
Determination of trace amount of Cu2+ with a multi-responsive colorimetric and reversible chemosenso
A novel fluorescent probe for copper ions based on polymer modified CdSe/CdS core/shell quantum dots
Fluorescent Determination of Trace Calcium in Water from High-Parameter Power Plant Based on a Porph
Simulation of 13C NMR Chemical Shifts of Carbinol Carbon Atoms by Using Quantitative Structure-spect
期刊信息
位置：