中文摘要：

目的观察左、右归丸对去卵巢大鼠骨髓间充质干细胞（BMSCs）增殖及成骨、成脂分化后凋亡蛋白表达的影响。方法 60只雌性SD大鼠,取40只切除双侧卵巢制备模型,随机均分为模型组（1.0 g/kg蒸馏水）、左归丸组（9.45 g/kg左归丸）、右归丸组（10.26 g/kg右归丸）和补佳乐组（0.09 mg/kg戊酸雌二醇）,另取10只作为空白组（1.0 g/kg蒸馏水）,10只双侧切除卵巢周围少量脂肪作为假手术组（1.0 g/kg蒸馏水）,各组灌胃给药12周后,处死大鼠,取股骨和胫骨体外培养BMSCs,采用MTT法检测细胞增殖,Western blot法观察半胱天冬氨酸蛋白酶-3（Caspase-3）和B淋巴细胞瘤-2基因（Bcl-2）的蛋白表达。结果 MTT法显示,左、右归丸对BMSCs增殖有促进作用,左归丸效果更佳。成骨分化后,与空白组相比,模型组Caspase-3蛋白表达上调（P〈0.05,P〈0.01）,Bcl-2蛋白表达下调（P〈0.01）;与模型组相比,左归丸组、右归丸组Caspase-3蛋白表达下调而Bcl-2蛋白上调（P〈0.05,P〈0.01）。成脂分化后,与空白组比较,模型组、左归丸组和右归丸组Caspase-3蛋白表达显著上调,Bcl-2蛋白下调（P〈0.05）,成骨、成脂分化后左归丸组与右归丸组比较均有显著性差异（P〈0.05）。结论左、右归丸均能抑制去卵巢大鼠BMSCs成骨、成脂分化后的凋亡,且左归丸有益于成骨分化,右归丸有益于成脂分化。

英文摘要：

AIM To observe the effects of Zuogui Pills and Yougui Pills on the proliferation of BMSCs and the expression of apoptosis after osteogenic and adipogenic differentiation in ovariectomized rats.METHODS Of sixty female SD rats,forty rats followed by bilateral ovariectomy were randomly divided into ovariectomy group （1.0 g/kg distilled water）,Zuogui Pills group （9.45 g/kg Zuogui Pills） and Yougui Pills group （10.26 g/kg Yougui Pills）and Bujiale group （0.09 mg/kg estradiol valerate）,another ten rats as control group （1.0 g/kg distilled water）,ten rats bilateral excision of a small amount of fat around the ovary was treated as sham operation group （1.0 g/kg distilled water）.After 12 weeks of administration,the rats were killed,BMSCs were cultured in vitro.Cell proliferation was detected by MTT assay.Western blot was used to detect the protein expressions of Caspase-3 and Bcl-2.RESULTS MTT assay showed that the proliferation of BMSCs were promoted in Zuogui and Yougui Pills groups,and the proliferation effect in Zuogui Pills group was better than that in Yougui Pills group.After osteogenic differentiation,as compared with the control group,the Caspase-3 expression of ovariectomy group was up-regulated （P〈0.05） and the expression of Bcl-2 was down-regulated （P〈0.01）.As compared with ovariectomy group,the expression of Caspase-3 was decreased and the expression of Bcl-2 was up-regulated in Zuogui Pills group and Yougui Pills group.After adipogenic differentiation,as compared with the control group,the expressions of Caspase-3 were significantly up-regulated and Bcl-2 were down-regulated in the ovariectomy group,Zuogui Pills group and Yougui Pills group （P〈0.05）.After osteogenic and adipogenic differentiation Zuogui Pills group and Yougui Pills group were significant different （P〈0.05）.CONCLUSION Zuogui Pills and Yougui Pills both can inhibit the apoptosis of BMSCs after osteogenic and adipogenic differentiation in ovariectomized rats.Zuogui Pills can promote BMSCs osteogene