中文摘要：

目的对1例入境的美国籍人类免疫缺陷病毒1型（HIV-1）感染者血液样本进行基因序列分析,鉴定其亚型。方法对该名HIV感染者进行流行病学调查,从其血浆样本中提取病毒总RNA,并应用巢式RT-PCR扩增HIV-1gag和pol基因,测序后通过系统进化分析进行亚型鉴定。结果基于gag和pol基因系统进化分析显示,该感染者携带的毒株基因序列均与HIV-1 CRF22_01A1亚型国际参考株聚集成簇,Bootstap值分别为74%和100%,证实其为HIV-1 CRF22_01A1亚型。这是国内首次报道检出HIV-1 CRF22_01A1亚型。结论 HIV-1 CRF22_01A1亚型毒株已随国际旅行者传入我国,应加强对国际旅行人员HIV的分子监测。

英文摘要：

Objective To identify the HIV-1 subtype of the plasma sample collected from a HIV infected American.Methods Epidemiology survey was conducted. The total RNA was extracted from plasma sample,and the HIV-1gag and pol gene were amplified by nest RT-PCR. The PCR products were sequenced and analyzed. Results The sample sequence clustered with HIV-1 CRF22_01A1 subtype international reference strains based on the phylogenetic analysis. The Bootstap values were 74% in gag gene and 100% in pol gene. It was showed that the sample was HIV-1 CRF22_01A1 subtype. Conclusion HIV-1 CRF22_01A1 subtype had been transmitted into China through international travellers, and it is important to strengthen the molecular surveillance on HIV among international travellers.