中文摘要：

①目的探讨丙泊酚（Propo{01）诱导脂多糖（LPS）刺激的树突细胞（DC）向分泌白细胞介素（IL）-10的树突细胞亚群-CD11clowCD45RBhighDC分化。②方法采用磁珠分选技术获得C57BL／6小鼠脾脏树突细胞，将提取的DC分为6个组，分别加入同体积的生理盐水（NS组），LPS1Hg／mL（L组），LPS1μg／mL＋5μg／mL丙泊酚（P1组）、LPS1μg／mL＋10μg／mL丙泊酚（P2组）、LPS1μg／mL＋20μg／mL丙泊酚（P3组）后进行培养24小时，分别用流式细胞仪检测各组细胞表面分子CD40、CD80、CD86、I-a／e的表达情况，采用ELISA法检测培养液中IL-10的含量。③结果与NS组相比，L组DC表面分子CD40、CD80、CD86和I-a／e的表达均明显增强（P〈0．05），同时IL-10的分泌升高；而与L组相比，较大剂量丙泊酚能显著降低DC表面分子CD80、CD40和I-a／e的表达并增强CD86的表迭及IL-10的分泌（P〈0．05），且-定的剂量效应关系。④结论丙泊酚可能通过介导IL-10的分泌促进DC向CD11clowCD45RBhighDC方向分化从而抑制早期炎症反应。

英文摘要：

Objective To explore the possibility of propofol on promoting DC to CDllcl~w CD4SRBhi~hDC subsets which produce IL-10. Methods Purification of splenic DCs cells in C57BL/6 mice were administrated by magnetic beads sorting.Then,these splenic DCs cells were divided into six groups：the NS group, LPS 1μg/mL（L group）, LPS 1μg/mL＋Propofol 5μg/mL （P1 group） ,LPS 1μg/ mL＋Propofol 10μg/mL （P2 group）, LPS1μg/mL ＋ Propofol 20μg/mL （P3 group）. Cultured after 24 hours,flow cytometry was used to determine expressions of DC surface molecules including CD40, CD80,CD86,I-a/e in six groups.IL-10 levels in DC culture supernatants were determined by sandwich enzyme-linked immunosorbent assays （ELISA）.Results Compared with the results of the NS group, the expression of CD40, CD80, CD86, I-a/e as well as IL-I0 secretion level was increased in the L group.Compared with the results of the L group, propofol could markedly decrease the expression of CD80,CD40 and I-a/e while enhancing the expression of CD86 as well as IL-10 secretion level.Besides, the enhancement of IL-10 secretion level hinges upon the rise of propofol concentration.Conclusion Propofol can inhibit the early inflammatory response by promoting DC to CDllc lowCD45RBhigh DCs differentiation and adjusting the secretion of IL- 10.