位置:成果数据库 > 期刊 > 期刊详情页
Genetic Analysis and Mapping of the Purple Gene in Purple Heading Chinese Cabbage
  • 时间:0
  • 分类:S634.1[农业科学—蔬菜学;农业科学—园艺学]
  • 作者机构:College of Horticulture, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China of Ministry of Agriculture,Northwest A & F University
  • 相关基金:supported by the National Science Foundation of China (Grant No. 31672163);the ‘863’ Plan of China (Grant No. 2012AA100105-5);the National Science and Technology Support Program of China (2014BAD01B0802)
中文摘要:

To analyze genetic linkage and map purple gene(Br Pur) controlling purple inner leaves trait of Chinese cabbage, a F2 population was constructed by selfing a F1 plant from homozygous purple heading line ‘14S839’ and homozygous orange heading line ‘14S162’. The phenotype investigation of head color showed that the segregation ratio of purple to non-purple individuals was consistent with the expected ratio of 3:1,which indicated that purple inner leaves trait is controlled by a single dominant gene. A total of 297 SSRs in whole genome of Chinese cabbage were tested by modified Bulked Segregant Analysis(BSA) method. Two linked markers flanking Br Pur, A710 and A714, were obtained and Br Pur was mapped on linkage group A07 based on the sequence of these two markers. Subsequently, two new markers flanking Br Pur, CL-12 and B214-87, were developed based on two known anthocyanins-related genes, Br4CL3 and Bra004214. Genetic mapping of all markers in the F2 mapping population showed CL-12 and B214-87 linked to Br Pur with the genetic distance of 3.1 c M and 3.5 c M, respectively.

同期刊论文项目
同项目期刊论文