中文摘要：

采用抗原决定基法制备了胰岛素电化学分子印迹传感器。以胰岛素C端多肽作为模板分子,定向自组装在Au电极上,以邻苯二胺为功能单体,电化学聚合制备分子印迹聚合膜。以Na OH为洗脱液,洗脱模板分子,形成的与胰岛素C端多肽三维结构相匹配的分子印迹孔穴能特异性识别胰岛素。重吸附胰岛素分子后,以K3[Fe（CN）6]/K4[Fe（CN）6]为探针,通过测量探针在电极表面产生的电流大小实现胰岛素的间接测定。在1.0×10-145.0×10-13mol/L浓度范围内,传感器的电流响应值与胰岛素浓度呈良好的线性关系,检出限为7.24×10-15mol/L（3σ）。此传感器具有较好的选择性和稳定性,并成功用于血清样品中胰岛素的测定。

英文摘要：

A novel molecularly imprinted electrochemical sensor for direct detection of insulin was prepared based on epitope imprinting. C-Terminal polypeptide in insulin as template molecule was firstly self-assembled on the Au electrode. Then the molecularly imprinted polymer（ MIP） was fabricated by electropolymerization with o-phenylenediamine（ o-PD） as functional monomer on this Au electrode. After elution of template molecules by Na OH solution,the imprinting cavities were formed with the three-dimensional structure matched with the polypeptide in insulin molecules. The imprinting cavities could specifically recognize and rebind with insulin molecules. With K3[Fe（ CN）6]/K4[Fe（ CN）6] as a probe,the insulin was indirectly detected.There was a linear relationship between the response current and the insulin concentrations in the range of1. 0 × 10-14-5. 0 × 10-13mol/L,and the detection limit was 7. 24 × 10-15mol/L. The developed sensor exhibited good selectivity and stability,and could be applied to the determination of serum samples.