中文摘要：

目的探讨广东汉族人群磷酸二酯酶4D基因（phosphodiesterase 4D,PDE4D）单核苷酸多态性（single nucleotide polymorphism,SNP）与缺血性脑卒中的关系。方法采用病例-对照的研究方法,选择广东地区712例缺血性脑卒中患者作为病例组,另选择同期健康体检的广东居民774例作为对照组。根据TOAST分型将病例组又分为大动脉粥样硬化型327例、小动脉闭塞型221例、心源性栓塞型54例、其他明确病因型52例和不明原因型58例。使用SNaPshot方法对PDE4D基因SNP83多态性进行检测,并分析SNP83多态性与缺血性脑卒中及其5个亚型之间的关系。结果病例组与对照组SNP83的CC、CT和TT基因型分布比较,差异无统计学意义（2.9%vs2.3%,29.1%vs 26.2%,68.0%vs 71.4%,P〉0.05）。在显性、隐性及加性3种遗传模型下,PDE4D基因SNP83多态性与病例组及5个亚型均无相关性。结论 PDE4D基因SNP83多态性与广东汉族人群缺血性脑卒中不相关。

英文摘要：

Objective To study the relation between single nucleotide polymorphism（SNP83）of phosphodiesterase 4D（PDE4D）gene and ischemic stroke（IS）in Guangdong Han population. Methods Seven hundred and twelve IS patients served as an IS group and seven hundred and seventy four residents undergoing physical examination served as a control group.The SNP83of PDE4Dwas detected by SNaPshot.The relation of SNP83of PDE4Dwith IS and its subtypes was analyzed.Results No significant difference was found in the distribution of SNP83CC,CT and TT genotypes between the two groups（2.9%vs 2.3%,29.1%vs 26.2%,68.0%vs 71.4%,P 〉0.05）.The SNP83of PDE4Dwas not significantly related with IS and its subtypes in dominant, recessive and additive models.Conclusion SNP83of PDE4Dis not related with IS and its subtypes in Guangdong Han population.