中文摘要：

目的 ：检测Yes相关蛋白（Yes-associated protein,YAP）在口腔鳞状细胞癌（oral squamous cell carcinoma,OSCC）中的表达及临床意义,判断其是否与肿瘤细胞的侵袭和迁移特性相关。方法：利用免疫组织化学染色检测68例原发口腔鳞癌患者组织标本中YAP的表达情况,分析其与各临床病理因素之间的关系;构建靶向YAP的sh RNA以及存在特定位点突变的YAP过表达载体,转染含YAP蛋白的TB2T4和SCC23口腔鳞癌细胞;利用细胞划痕实验和细胞侵袭实验检测YAP干扰及过表达对肿瘤细胞迁移和侵袭能力的影响。采用SPSS18.0软件包对数据进行统计学处理。结果：在原发口腔鳞癌组织中,YAP在细胞核和胞质的表达均显著高于正常口腔黏膜上皮组织（P〈0.05）。YAP在细胞核的表达情况与患者颈淋巴结转移（P〈0.001）及5年总体生存率（P=0.008）显著相关。沉默YAP基因后,TB2T4和SCC23细胞的迁移和侵袭能力受到显著抑制（P〈0.05）;过表达YAP基因后,肿瘤细胞的迁移和侵袭能力得到部分恢复（P〈0.05）。结论：YAP的表达与口腔鳞癌患者颈淋巴结转移及预后相关,YAP在口腔鳞癌细胞的侵袭、迁移中可能发挥重要作用。

英文摘要：

PURPOSE： To investigate the expression of YAP（Yes-associated protein） in oral squamous cell carcinoma（OSCC） and determine if YAP was associated with tumor cell invasion and migration. METHODS： Sixty-eight primary OSCC patients were enrolled in this study. YAP expression in tissue specimens was investigated by immunohistochemistry staining. YAP putback plasmid with specific mutation sites after YAP-sh RNA interference was constructed by site-directed mutagenesis. Cell scratch and cell invasion assay were performed after YAP-si RNA interference or YAP putback again in TB2T4 and SCC23 cells. The data was analyzed using SPSS18.0 software package. RESULTS： Nuclear and cytoplasmic expression of YAP was increased in primary OSCC tissue samples（P〈0.05）. Nuclear expression of YAP was associated with cervical lymph node metastasis（P〈0.001） and 5-year overall survival rate（P=0.008）. YAP knockdown greatly suppressed tumor cell migration and cell invasion both in TB2T4 and SCC23 cells（Scramble vs YAP-sh RNA, P〈0.01）, but these abilities were rescued subtotally when YAP was put back to the YAP-sh RNA stable cells（Control vs YAP putback, P〈0.01）. CONCLUSION： YAP expression in OSCC was associated with cervical lymph node metastasis and YAP might play an essential role on the invasion ability of OSCC cells.