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鸽子对流感病毒的易感性和传播性研究
  • 期刊名称:国际流行病学传染病学杂志
  • 时间:0
  • 页码:4-8
  • 语言:中文
  • 分类:R511.703[医药卫生—临床医学;医药卫生—内科学] R512.91[医药卫生—临床医学;医药卫生—内科学]
  • 作者机构:[1]浙江省医学科学院生物工程研究所,杭州310013, [2]北京市农林科学院畜牧兽医研究所,100089
  • 相关基金:国家自然科学基金(30872163)
  • 相关项目:流行性感冒病毒跨种间传播与感染机制的研究
中文摘要:

目的探讨鸽子对流感病毒的易感性和传播性。方法通过数据统计、动物感染实验、血液凝集试验、原位组织植物凝集素结合试验、原位组织病毒结合实验、组织细胞糖链受体提取及其病毒结合试验等,从多方面开展鸽子对流感病毒的易感性和传播性的研究。结果流行病学调查和动物感染实验证实鸽子传播流感病毒可能性极少,原位组织植物凝集素结合试验和原位组织病毒结合实验确定了病毒感染部位是鸽子的肠道上皮黏膜细胞,植物凝集素结合试验的结果提示鸽子组织细胞带有不适合流感病毒唾液酸酶作用的糖链,组织细胞糖链受体提取物的病毒结合试验已发现有病毒结合带,根据病毒结合实验也发现鸽子仅对禽类流感病毒敏感。结论鸽子能被禽流感病毒感染,但不会被人流感病毒感染,鸽子不会传播流感病毒。

英文摘要:

Objective Research on the susceptibility and the transmissibihty of domestic pigeons to influenza viruses. Method Through data statistics, animal infection experiment, hemagglutinafion assay, lectin detecting assay in situ, virus-binding assay in situ, isolation of receptoral glycoconjugates and TLC/virus-binding assay etc, to ascertain the above research. Results Both epidemiolodgical research and the animal infection experiment demonstrated that pigeon had not transmissibility for influenza virus. Both lectin detecting assay and virus-binding assay in situ expressed that the infective position of pigeon was the epithelial cells in intestine tract. The data from phytohemagglutinin assay indicated that the sialyl-glycoconjugates on the cell membrane of pigeon did not adapted for hydrolysis of neuraminidase from influenza virus. The band of influenza virus binding in acidic sialyl-glycolipids was found with the TLC/virus-binding assay, and only was bound by avian-like virus (A/PR/8/34 strain), not by human-like virus (A/Memphis/1/71 strain).Conclusions Pigeon is sensitive to infection of avian influenza virus, but not to human isolates. Pigeon almost could nottransmit for influenza virus.

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