中文摘要：

Bcl-2是与细胞凋亡相关因素的重要成员之一,是目前研究细胞凋亡的热点.本研究根据GenBank中已发表的鸡β-actin和Bcl-2基因序列设计引物,成功建立了检测鸡Bcl-2 mRNA表达的荧光定量PCR方法,并成功应用于检测益生菌对传染性法氏囊病（IBD）疫苗免疫后的雏鸡免疫器官中Bcl-2 mRNA表达量的变化,结果显示益生菌联合IBD疫苗免疫雏鸡后,其免疫器官中Bcl-2 mRNA表达量高于IBD疫苗单独免疫组.此方法具有快速、高通量、线性范围广、特异性强、灵敏度高等特点,为进一步定量研究鸡Bcl-2 mRNA表达及其与相关疾病的关系奠定了技术基础.

英文摘要：

Bcl-2 is an important member of the apoptosis-related factors,which has been a hot topic of apoptosis research currently. According to the chicken β-actin gene and Bcl-2 gene sequences published in GenBank,fluorescent quantitation PCR for detecting the expression of Bcl-2 mRNA in chicken was successfully established, and which was applied to detecting the expression of Bcl-2 mRNA in immune organs after immunized with probiotics and infectious bursal disease （IBD vaccine）, The results showed that the expression of Bcl-2 mRNA in immune organs was higher than IBD vaccine immunization alone group. This method was characterized by rapidity, high-flux, wide linear range, good specificity and high sensitivity. So this method would provide the basis for making further efforts for quantitative research on the expression of Bcl-2 mRNA and laying technical foundation for the related diseases study.