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中国大蒜种质资源遗传多样性和群体遗传结构分析
  • ISSN号:0578-1752
  • 期刊名称:中国农业科学
  • 时间:2012.8.8
  • 页码:3318-3329
  • 分类:S435.111.4[农业科学—农业昆虫与害虫防治;农业科学—植物保护]
  • 作者机构:[1]中国农业科学院蔬菜花卉研究所,中国北京100081, [2]USDA-ARS Vegetable Crops Research Unit and Department of Horticulture, University ofWisconsin, 1575 Linden Drive, Madison, Wisconsin 53706, USA
  • 相关基金:公益性行业(农业)科研专项(200903018-3)、国家青年科学基金项目(31000910)、国家基础性专项基金(2005DKA21002-33)、农业部园艺作物生物学与种质创制重点实验室项目和教育部留学基金专项奖学金(留金出[2009]3012号)
  • 相关项目:中国大蒜资源遗传多样性和蒜氨酸酶基因SNP检测及关联作图
中文摘要:

【目的】从分子水平了解中国大蒜种质资源的群体遗传结构和遗传背景。【方法】利用AFLP、SSR和InDel这3种分子标记对国家无性繁殖蔬菜资源圃保存的212份大蒜资源进行检测,通过Mega软件进行最大相似性聚类分析,structure2.1软件进行群体遗传结构分析,SSPS软件进行分子标记与大蒜辣素含量和21个数量性状进行一元线性模型检测,考察两者之间的关联性及群体遗传结构的影响。【结果】3种分子标记在212份种质中扩增出502个位点,多态性位点为492个。群体遗传结构与聚类分析均将所有资源划分为5个群体,划分的类别基本一致。然而,群体遗传结构分析划分的5个群体,群内遗传信息多样性指数较小。对212份种质的22个数量性状与分子标记的线型模型分析表明,包括大蒜辣素含量在内的多个数量性状受群体遗传结构的影响较小。【结论】中国大蒜种质资源遗传背景丰富,群体遗传结构对数量性状的分布影响较小,适合进一步进行性状与分子标记之间关联分析研究。

英文摘要:

[Objective] The objective of the experiment is to understand the diversity and genetic structure of garlic germplasm resources in China. [Method] Two-hundred and twelve accessions of garlic germplasm preserved in China were detected and analyzed based on AFLP, SSR and InDel. Neighbor-joined clustering was analyzed by soft'ware Mega, and the population genetic structure was studied by software Structure 2.1. The effect of genetic structure on association analysis between the molecular markers and 21 quantitative traits including allicin content was evaluated by general linear model (GLM) by software S SPS 13.0. [ Result ] Totally, 502 allels were amplified by AFLP, SSR and InDel primers, and 492 of them were polymorphic among 212 accessions of garlic. All accessions were divided into 5 groups by both structure analysis and neighbor-joining clustering. However, the Shannon's index of each group assumed by genetic structure analysis was smaller than that assumed by neighbor-joining clustering, which indicated that the genetic structure analysis could interpret genetic relationship among the individual accessions in more details. Most of traits including allicin content were slightly affected by population structure, which indicated that the germplasm in this study was acceptable to be the populations for association mapping. [ Conclusion] The garlic germplasm resources in China possess a great diversity. The population structure of the garlic germplasm resources slightly affect the quantitative traits including the allicin content, which means that the population of the garlic germplasm is a potential candidatenatural population for association analysis between the molecular markers and the quantitative traits.

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期刊信息
  • 《中国农业科学》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院 中国农学会
  • 主编:万建民
  • 地址:北京中关村南大街12号中国农业科学院图书馆楼4101-4103室
  • 邮编:100081
  • 邮箱:zgnykx@caas.cn
  • 电话:010-82109808 82106279
  • 国际标准刊号:ISSN:0578-1752
  • 国内统一刊号:ISSN:11-1328/S
  • 邮发代号:2-138
  • 获奖情况:
  • 中国期刊方阵“双高”期刊,第三届中国出版政府奖提名奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:85620