中文摘要：

为探究COR基因在油菜抵御逆境胁迫中的作用,采用RACE技术从白菜型油菜‘陇油6号’克隆获得1个新的COR15-like基因BrCOR15,该基因全长616bp,其中,5′非翻译区59bp,3′非翻译区167bp,开放阅读框390bp,编码129个氨基酸,推测其蛋白分子质量为13.9ku,理论等电点为5.2。该基因编码蛋白质的氨基酸序列与多种植物具有较高一致性,与拟南芥AtCOR15、AtCOR15a,高山离子芥CbCOR15,高山葶苈DaCOR15a、DaCOR15b的氨基酸序列一致性分别为74.6%、77.7%、72.3%、77.9%和76.7%。实时荧光定量PCR分析表明,BrCOR15基因在油菜的茎、叶和下胚轴均有表达,无组织特异性;该基因表达受低温、ABA和H2O2诱导,与单独处理结果相比,经MAPKK抑制剂U0126预处理12h再经低温、ABA和H2O2处理后,BrCOR15基因的表达量明显降低,表明该基因在油菜适应低温、ABA和H2O2胁迫过程中发挥作用。

英文摘要：

In order to explore the role of COR gene from Brassica campestris under resisting stress conditions,a novel COR15-like gene BrCOR15 was isolated from Brassica campestris ＇Longyou 6＇ by RACE. The full-length cDNA of BrCOR15 was 616 bp,which contained a 5＇-UTR of 59 bp,a 3＇ UTR of 167 bp,anda390 bpopeningreading frame. The deduced protein was 129 amino acids with molecular weight 13.9 ku and isoelectric point 5.2. At the amino acid sequence level, the encoded protein of BrCOR15 showed high identities with AtCOR15, AtCOR15a, CbCOR15, DaCOR15 and DaCOR15b （74.6%,77. 7%,72. 3%,77. 9% and 76. 7%）. RT-PCR analysis revealed thatBrCOR15 was expressed in the stems,leaves,and hypocotyls,mostly,it had no tissue specificity. The transcript level of BrCOR15 was increased in response to cold, ABA and H2O2 stress. However, MAPKK inhibitor U0126 was pretreated 12 h, and then treated by ideceasing temperature, ABA and H2O2 stress, and compared with the single stress the results indicated that the expression level of BrCOR15 significantly decreased. The results suggested that BrCOR75 plays an important role during cold, ABA and H2O2 stress in Brassica campestris.