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中文摘要：
将猪松质骨脱脂脱蛋白、煅烧后再进行球磨制备羟基磷灰石（HA），与壳聚糖（CS）共混制备成HA／cs复合膜。通过正交设计考察了球磨条件对HA粒径的影响，在复合膜上接种MC3T3-E1前体成骨细胞，采用SEM及四甲基偶氮唑盐（MTT）方法检测细胞在膜表面的形态及增殖，评价了材料的细胞相容性。结果表明，所制备HA为粒径较均一的微米级球形粒子，中值粒径D50为1．21～1．67μm。共混膜内HA在基质中分布均匀，二者结合紧密，复合膜具有较好的力学性能，MC3T3-E1细胞能在复合膜上很好地黏附生长。细胞增殖结果表明，复合膜制备条件煅烧温度为1000℃，球配比4：4：2，球磨速率为230r·min-1，球磨时间为2．5h，HA：CS=5：5（质量比）时最利于细胞增殖。
英文摘要：
Hdroxyapatite（HA） was prepared using porcine trabecular bone after being degreased, deproteined, calcined and ball milling. HA/chitosan（CS） composite membranes were prepared by blending and drying HA and CS solution. The effect of milling conditions on the HA particle size was inspected by the orthogonal design. MC3T3- E1 preosteoblasts were seeded on the surface of the composite membranes, and then cell morphology and proliferation were detected by SEM and 3 - （4,5 - dimethyl- 2 - thiazolyl） - 2,5 - diphenyl tetrazolium bromide（MTT） methods. The results show that the prepared HA is relatively homogeneous micrometer-scale spherical particle with median diameter（Dso） in 1.21 ~ 1.67 tim range, and HA particles are distributed uniformly in the matrix, HA particle and CS combined closely. Composite membrane has good mechanical properties, MC3T3 - E1 cells can adhere and grow well on the surface of composite membrane. The cell proliferation results show that composite membrane under the preparation conditions： calcination temperature 1000 C, the milling ball propotion 4 ： 4 ： 2, milling rate 230 r min 1 , milling time 2. 5 h, HA . CS = 5 ： 5 （mass ratio） is the most beneficial for cell proliferation.
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