中文摘要：

瞄准：在 hepatocellular 在 T 助手(Th ) 房间子集上调查不同麻药技术的影响癌(HCC ) 病人经历 hepatectomy。方法：收到了 hepatectomies 的 61 个 HCC 病人被使随机化进硬膜上的联合一般麻醉(G + E；n = 31 ) 或一般麻醉(G；n = 30 ) 组。血样品被获得在操作(d0 ) 前的早上，并且在上第二(d2 ) 并且第七(d7 ) 在操作以后的白天。Th 房间内容用流动 cytometry，即时反向的抄写聚合酶链反应和连接酶的 immunosorbent 试金被评估。结果：在所有 61 个病人， Th1 和 Th2 房间频率，和干扰素 --(IFN-) mRNA 表示显著地在 d2 上增加了，与 d0 相比。他们在 d7 上稍微恢复了，并且 Th1/Th2 比率在 d7 上显著地增加了，与 d2 相比。相反， Th17，规章的 T 房间(Treg ) ，和 interleukin-17 (IL-17 ) 层次和 FOXP3 mRNA 表示没在 d2 上显示出重要变化，然后显著地在 d7 上减少了。同样， d2 上的血浆 IFN- 集中在 d0 上比那高得多，然后部分在 d7 上恢复了。作为与 G 组相比，在 G + E 组， Th1 房间频率和 Th1/Th2 比率在 d7 上更高在 d2 上并且显著地是稍微更高的当 Th2， Th17，和 Treg 房间频率在 d2 上是稍微更低的，并且显著地在 d7 上降低时。在 d7 上，一致地，在 G + E 的 IFN- mRNA 和蛋白质层次和 IFN-/IL-4 比率组织比在 G 组的那些高。相反， IL-17 mRNA 水平和在 G + E 组的转变生长 factor-1 集中是比在 G 组的那些低的。结论：G + E 比在当减少时，向 Th1 转移 Th1/Th2 平衡的 G 优异 Th17 和 Treg，由支持反肿瘤 Th 的潜在地受益的 HCC 病人极化。

英文摘要：

AIM：To investigate the impact of different anesthetic techniques on T-helper（Th） cell subsets in hepatocellular carcinoma（HCC） patients undergoing hepatectomy.METHODS：Sixty-one HCC patients who received hepatectomies were randomized into an epidural combined general anesthesia（G ＋ E;n = 31） or a general anesthesia（G;n = 30） group.Blood samples were obtained the morning before the operation（d0）,and on the second（d2） and seventh（d7） day after the operation.Th cell contents were evaluated using flow cytometry,realtime reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay.RESULTS：In all 61 patients,Th1 and Th2 cell frequencies,and interferon-（IFN-） mRNA expression markedly increased on d2,compared to d0.They recovered slightly on d7,and the Th1/Th2 ratio increased markedly on d7,compared with d2.In contrast,Th17,regulatory T cell（Treg）,and interleukin-17（IL-17） levels and FOXP3 mRNA expression showed no significant change on d2,and then markedly decreased on d7.Similarly,plasma IFN-concentration on d2 was much higher than that on d0,and then partly recovered on d7.As compared with the G group,in the G ＋ E group,Th1 cell frequencies and the Th1/Th2 ratio were slightly higher on d2 and significantly higher on d7,while Th2,Th17,and Treg cell frequencies were slightly lower on d2,and significantly lower on d7.Consistently,on d7,IFN-mRNA and protein levels and the IFN-/IL-4 ratio in the G ＋ E group were higher than those in the G group.In contrast,the IL-17 mRNA level,and IL-17 and transforming growth factor-1 concentrations in the G ＋ E group were lower than those in the G group.CONCLUSION：G ＋ E is superior to G in shifting the Th1/Th2 balance towards Th1,while decreasing Th17 and Treg,potentially benefiting HCC patients by promoting anti-tumor Th polarization.