中文摘要：

目的研究锌转运蛋白3（ZnT-3）在APP／PS1转基因小鼠大脑皮质及海马内的分布和表达变化。方法应用免疫组织化学技术检测ZnT-3在APP／PS1转基因小鼠脑内的分布，并应用Western blot方法分析ZnT-3在大脑皮质及海马的表达水平。结果ZnT-3主要分布于APP／PS1转基因小鼠脑内的老年斑中，且主要定位于老年斑周围变性的神经元及其突起内；ZnT-3在APP／PS1转基因小鼠大脑皮质和海马的表达均明显高于野生型小鼠。结论ZnT-3在APP／PS1转基因小鼠大脑内的高表达以及在老年斑内的定位，提示ZnT-3可能在老年斑内锌离子的聚集过程中起着重要的调节作用，进而参与了APP/PS1转基因小鼠大脑内Aβ老年斑的形成。

英文摘要：

Objective To investigate the expression change and distribution of zinc transporter-3 （ZnT-3） in the hippocampus and cortex of APP/PS1 transgenic mouse, Methods The distribution of ZnT-3 in the APP/PSI transgenic mouse brain was analyzed with immunohisto-chemistry,and Western blot technique was used to analyze the expression level of ZnT-3 in the hippocampus and cortex of APP/PS1 transgenic mouse. Results ZnT-3 mainly distributed in the senile plaques in the APP/PS1 transgenic mouse brain. ZnT-3 immunoreactivity was strong in the degenerating neurites in the peripheral of the plaque. Western blot results showed high expression of ZnT-3 in the cortex and hippocampus of APP/PS1 transgenic mouse compare with wild-type mouse. Conclusion The overexpression of ZnT-3 and localization of ZnT-3 in the plaques of the APP/PS1 transgenie mouse brain suggest the important role of ZnT-3 in regulating zinc accumnlation during the pathological process of AD.