中文摘要：

[目的]探讨大剂量经口纳米粒径二氧化钛（nano-TiO2）和三氧化二铝（nano—Al2O3）粉染毒对小鼠血清乳酸脱氢酶（LDH）和α-羟丁酸脱氢酶（α—HBDH）活力的影响。[方法]分男4以5g/Kg体重剂量的微米（micro—TiO2）和nano-TiO2或-Al2O3粉给小鼠一次经口灌胃，14d后处死取血分离血清，分析LDH和α-HBDH活性变化。[结果]nano—Al2O3染毒小鼠血清LDH和α-HBDH活力比对照组明显降低（P〈0．05），但与micro—AL2O3染毒组相比差异无统计学意义（P〉0．05）；nano—TiO2染毒小鼠血清LDH活力比对照组有明显升高（P〈0．05），micro—TiO2染毒组小鼠血清LDH活力明显低于对照组和nano-TiO2染毒组（P〈0．05）；nano-TiO2染毒小鼠血清α—HBDH活力与micro—TiO2染毒组相比差异无统计学意义（P〉0．05）。[结论]nano-TiO2和nano—Al2O3粉对小鼠血清LDH和α-HBDH活性的影响明显不同，nano—TiO2和nano—Al2O3粉对小鼠血清LDH和α-HBDH活性的影响与相应的micro—TiO2和micro—Al2O3有明显差别。血清LDH和α-HBDH可能不一定适合于纳米金属材料的生物毒性评估。

英文摘要：

[Objective] To explore the effect of excess nano-particle alumina oxide and titanium dioxide on serum Lactate dehydrogenase （LDH） and α-Hydroxybutyrate Dehydrogenase （α-HBDH） activity in mice. [Methods] The Mice were treated with micro-or nano-particle alumina oxide or titanium dioxide powder at a single oral dose of 5 g/Kg body weight. 14 days later, the mice were sacrificed and the serum was collected for LDH and α-HBDH analysis. [ Results] The serum LDH and HBDH activity in Nano-Al2O3 group mice were lower significantly than that in control group but without obvious difference with that in micro-Al2O3 group mice. The serum LDH activity of nano-TiO2 group mice was significandy higher than that of control group mice, but there was no any difference in serum HBDH activity between micro-and nano-TiO2 group mice. The serum LDH activity in micro-TiO2 group mice decreased significandy than control group and nano-TiO2 group; there was no any difference in serum HBDH activity among the control, nano- and micro-TiO2, group mice. [Conclusions] There are significant different effects on serum LDH and α-HBDH between excess Nano-Al2O3 and Nano-TiO2 treated mice, excess nano-Al2O3 or nano-TiO2 has obvious different effect on serum LDH and α-HBDH than micro-Al2O3 or micro-TiO2, serum LDH and HBDH activity are probably not suitable for the evaluation on the biological injury and toxic of nano metal materials.