中文摘要：

为探讨BCG初次免疫，结核分枝杆菌Ag85A DNA疫苗加强免疫的序贯免疫策略对小鼠的免疫效果。采用BCG及结核分枝杆菌Ag85A DNA疫苗依次免疫小鼠，在末次免疫后的4、6、8周通过检测小鼠血清总IgG抗体、特异性淋巴细胞增殖．细胞因子的水平，观测BCG初次免疫，Ag85A DNA疫苗加强免疫的策略对小鼠的免疫效果。结果显示，采用BCG初次免疫，结核分枝杆菌Ag85A DNA疫苗加强免疫的策略组的小鼠与其它免疫方式组相比，IgG明显升高（P〈0．05）、特异性淋巴细胞明显增殖、IFN7、IL-2、IL-4水平明显增高（P〈0．05）。由此可知，在采用BCG初次免疫，结核分枝杆菌Ag85A DNA疫苗加强的免疫策略后，能增强对小鼠的免疫效应，尤其是Thl型细胞免疫反应增强明显，为进一步在动物体内进行保护性效应试验的研究提供了实验依据。

英文摘要：

To investigate the immune efficacy of BCG/pcAg 85A DNA vaccine prime-boost regimen against Mycobacterium Tu- berculosis. BALB/c mice were divided into PBS negative control and 3 immunity groups： BCG group, pc Ag 85A group BCG/ pcAg 85A group. All mice received three immunizations at 2-week interval. Specific IgG antibody in serum of mice was deter- mined with indirect ELISA in 4,6,8 weeks respectively after final vaccination. The splenic lymphocytes of mice were separated and stimulated with PPD to measure their proliferation and to evaluate the production of IFN-γ,IL-2 and IL-4 in cell suspen- sions of splenic cells by ELISA. Compared with the other three groups, the specific antibody levels against PPD, the stimulation index（SI）of splenic lymphocytes, IFN-7, IL-2 and IL 4were all statistically higher in BCG/pcAg 85A group （P〈 0. 05）. The BCG/pcAg 85A regimen for tuberculosis prevention in mice can stimulate the specific antibody and induce cellular immunity. The immune efficacy of the BCG/pcAg 85A regimen is slightly better than that of BCG and DNA vaccine.