中文摘要：

以苜蓿品种甘农1号下胚轴为外植体,研究不同培养基对苜蓿愈伤组织、体细胞胚形成的影响,通过体细胞胚发生途径建立再生体系,在此基础上,摸索农杆菌介导小反刍兽疫病毒F基因的转化条件。结果表明：所选培养基愈伤组织诱导率均为100%,UM＋0.1mg/L NAA＋0.5mg/L KT为诱导体细胞胚的最佳培养基。农杆菌介导的苜蓿遗传转化条件为：下胚轴预培养3d,农杆菌菌液侵染15min,然后在培养基上铺一层灭菌滤纸共培养3d后清洗,选择压为卡那霉素（Km）75mg/L,抑菌浓度为头孢霉素（Cef）300mg/L。本研究为制备防治小反刍兽疫转基因植物疫苗奠定了基础。

英文摘要：

Regeneration system of alfalfa cultivar Gan-nong No.1 was established through somatic embryogenesis.Callus and somatic embryo were induced by different medium and by hypocotyl as explants.Based on this system,Alfalfa transformation Condition by Agrobacterium-mediated with F gene of peste des petits ruminants virus（PPRV） was optimizated.The results showed that all of the probability of callus induced by different medium was 100%,and the best medium which can induce somatic embryo was UM＋0.1mg/L NAA＋0.5mg/L KT.The transformation system of alfalfa by Agrobacterium-mediated with F gene of PPRV was that pre-cultivated for 3 days,the hypocotyl was immersed in the bacterial suspension for 15 minutes and then cocultivated on MS medium covered with a piece of sterile filter paper for 3 days before washing,concentration of Km and Cef was 75mg/L and 300mg/L respectively.This study will lay the foundations for the preparation of transgenic plant vaccine which can prevent and cure PPR.