化学发光法灵敏度高、背景干扰小,尤其适合于复杂体系中微量被测物的分析。吖啶铺、鲁米诺、过氧草酸酯、1,2-二氧杂环丁烷等是应用广泛、量子产率较高的化学发光试剂。介绍了它们及其衍生物的结构修饰与化学发光性能研究进展,并展望了化学发光试剂修饰的前景。
Chemiluminescent assay showing advantages of high sensitivity and low background is suitable for trace analysis for complex systems. The development of new chemiluminescent molecules such as acridinium, luminol, peroxyoxalate and their derivatives and 1,2-dioxetane is reviewed.