中文摘要：

以尿酸是昆虫氮代谢的最终产物的理论为依据,采用高效液相色谱法测定小麦粉中害虫感染后的尿酸含量,从而对小麦粉的污染程度作出判断,并提高检测的灵敏度.结果表明,利用高效液相色谱法对尿酸标准溶液的检出限为1μg/m L,面粉中尿酸的最低检测限为1.48μg/g;赤拟谷盗成虫感染的小麦粉中检测出尿酸的含量高于幼虫感染的,锯谷盗成虫感染的高于幼虫感染的,而且检测小麦粉中尿酸含量与害虫虫口密度-感染时间呈很好的相关关系;赤拟谷盗成虫感染时间与虫口密度和检测出尿酸的含量相关关系回归方程为Z=10.35＋4.42X＋6.11Y＋1.32XY-0.06X2＋1.06Y2.在面粉厂随机取7份小麦粉样品进行尿酸的检测,检测出尿酸含量与根据回归方程和害虫发生实际情况预测的尿酸含量相比,准确率在83.00%-99.60%,说明可以利用高效液相色谱方法检测小麦粉中尿酸的含量来预测面粉中害虫的感染程度.

英文摘要：

Rapidly detecting insects in wheat flour has important practical significance to enhance edible quality and nutritional value of the wheat flour, and to improve export trade. Based on theory that uric acid is final product of insect nitrogen metabolism, high performance liquid chromatography （HPLC） was used to determine uric acid levels of wheat flours infested by pest insects and to make judgment insects infestation degree. The purpose of this paper was to develop the rapid and high sensitivity detection method for insect infested flour. The results showed that the detection limit of uric acid standard solution of HPLC method was 1μg/mL, and the minimum detection limit of uric acid in flour was 1.48 μg uric acid/g. The uric acid content in wheat flour infested by Tribolium castaneum adults was higher than that by larvae and the same as Oryzaephilus surinamensiz. Good correlation existed among uric acid content of infested wheat flour, population density and insect infection time. For red flour beetle adults, there was a regression equation between uric acid content （Z） and popualtion density （X）, infestion time （Y）： Z=10.35＋4.42X＋6.11Y＋1.32XY-0.06X2＋l.06Y2, Seven samples were randomly taken from mills for detection of uric acid. The accuracy rates about uric acid content between detected value and predicted value with actual insect number and regression equation were from 83.00% to 99.60%. All the results indicated that the HPLC method could be used for predicting insect infection degree by detecting uric acid levels in wheat flour.