中文摘要：

八角莲为中国特有珍稀植物,有很高的药用价值,其所含的鬼臼毒素是具有一定药效活性的环木脂内酯,具有杀虫、抗风湿、抗病毒、抗肿瘤等活性.试验以八角莲叶、叶柄、根为外植体诱导愈伤组织,将组培苗炼苗移栽,成功建立八角莲的组培-移栽体系,同时采用高效液相色谱技术（HPLC）测定并比较八角莲3种愈伤组织、组培苗和野生植株中鬼臼毒素的含量差异.结果显示,以八角莲叶、叶柄、根为外植体,成功诱导出3种愈伤组织;野生八角莲叶中鬼臼毒素含量最高为（7.27±0.75）mg·g^-1DW;愈伤组织培养60d,鬼臼毒素含量为（1.98±0.32）mg·g^-1DW.通过组织培养可在不破坏野生资源的前提下生产鬼臼毒素,从而为开发鬼臼毒素类抗癌药物提供大量原料.

英文摘要：

Dysosma with high medicinal value is endemic and rare plants in China, Podophyllotoxin is cyclolignolide that has some pharmacological activities, and it is known to have various functions and bioactivities such as insecticide, anti-rheumatism, antivirus and antitumor. To establish the tissue culture-transplanting system of Dysosma versipellis （Hanee） M. Cheng, the podophyllotoxin contents in callus, tissue culture seedling and wild D. versipellis were compared. The leaves, petioles, roots of D. versipeUis used as explants to induce callus, and the seedlings were acclimatized and transplanted. The podophyllotoxin content in three types of callus, tissue culture seedling and wild D. versipellis were determined with HPLC. The results show that three types of callus were induced with leaves, petioles and roots of D. versipellis successfully. The highest level of podophyllotoxin was （7.27 ± 0.75 ） mg · g^-1DW in the leaf of wild D. versipeUis. The content of podophyllotoxin was （ 1.98 ±0.32） mg · g^-1DW after cultivated 60 d. With tissue culture, the podophyllotoxin can be produced without wild resources damage and a range of raw materials can be supplied for the development of podophyllotoxin anticancer drugs.