中文摘要：

目的探讨富含亮氨酸重复序列免疫球蛋白样蛋白1（LRIG1）基因与表皮生长因子受体（EGFR）在人脑胶质瘤细胞中的相互作用及其对肿瘤细胞生长影响的机制。方法用免疫共沉淀法检测人脑胶质瘤细胞系GL15中LRIG1与EGFR的相互作用。构建针对LRIG1基因的干扰片段及非特异性的对照片段分别转染GL15细胞系，G418（600mg／L）筛选出稳定株，Westernblot法检测LRIG1表达的改变及其对EGFR表达的影响；噻唑蓝（MTT）比色法检测LRIG1表达下调对胶质瘤细胞增殖的影响。结果免疫共沉淀法表明胶质瘤细胞系GL15中LRIG1与EGFR存在相互作用。成功构建了针对LRIG1基因的特异性shRNA表达载体，转染细胞后LRIG1基因表达下降54．7％；同时干扰组细胞EGFR蛋白水平与阴性对照组比较上升了57．1％。MTY结果显示干扰组细胞增殖率高于对照组。结论LRIG1通过与EGFR结合发挥抑癌作用，干扰LRIG1表达可削弱这种作用，导致肿瘤细胞增殖加速。

英文摘要：

Objective To investigate the interaction between LRIG1 gene and epidermal growth factor receptor （EGFR） in human glioma ceils and explore the action mechanism of effects of the two genes on growth of glioma cells. Methods Co-immunoprecipitation was performed to detect the interaction of LRIG1 and EGFR in human glioma cell line GL15. Small interfering RNA （siRNA） targeting LRIG1 gene and negative shRNA （Meg） were constructed and transfected into GL15 glioma cells. And the cells （siRNA） that stably suppressed LRIG1 expression were selected by G418 （600 mg/L）. The change of LRIG1 protein level was measured by Western blot, and growth curves were determined by MTr assay. Results The interaction of LRIG1 and EGFR in human glioma cell line GL15 was confirmed by co-immunoprecipitation, siRNA targeting LRIG1 gene and negative shRNA （Meg） were successfully constructed. The LRIG1 protein level in pGenesil2-LRIG1-shRNA （siRNA） transfected cells was significantly silenced by 54.7% , and the EGFR protein level increased by 57.1%. The siRNA cells had higher proliferation rate than controls. Conclusion The LRIG1 protein inhibits tumor generation and proliferation by interacting with EGFR, which was weakened by interfering LRIG1 expression,leading to the promotion of tumor prolif eration.