目的:建立骨碎补药材脂溶性成分的气相色谱(GC)指纹图谱。方法以环己烷为提取溶剂,经索氏提取法获得骨碎补药材的脂溶性成分。采用DB-5毛细管柱,程序升温,并利用主成分分析法(PCA )对指纹图谱进行统计分析。结果利用PCA对GC指纹图谱进行处理,骨碎补药材的正伪品可明显区分。结论GC指纹图谱为骨碎补药材的进一步质量控制提供了有用的参考。
Objective To study the gas chromatographic (GC) fingerprint of Rhizoma Drynariae .Methods The samples were placed in a Soxhlet′s extractor with cyclohexane and refluxed for 3 hours .The GC conditions were as follows :a DB-5 column ,programmed column temperature ,FID detector .Results Significant differences were observed between genuine and fake samples in the principal components analysis (PCA ) score plot .Conclu-sion This method provides useful reference for quality control of Rhizoma Drynariae .