中文摘要：

背景：有全身性红斑狼疮的病人经常有各种各样的自身抗体。在这些抗体之间的关系仍然糟糕被理解。现在的学习的目的是观察到 anti-SSB 抗体和 anti-dsDNA 抗体生产与合成 SSB 肽介绍后面的免疫独自一个的，独自一个的 DNA 或有这二抗原的合作免疫。方法：SSB 214 - 225 一肽被有机化学综合稳固阶段的肽合成。兔子与下列抗原被使免疫：与锁眼恋栈者血青蛋白(KLH ) 连接的合成 SSB 肽， DNA， SSB 正 dsDNA， KLH 和 PBS。抗体被 ELISA 测量。组织病理学说和直接 immufluorescence 试金也被使用。结果：Anti-SSB 和 anti-dsDNA 抗体被生产分别地与 SSB 肽和 DNA 跟随免疫。在合作免疫组的 SSB 抗体的水平比 SSB 肽免疫组的高。然而，在合作免疫组的 anti-dsDNA 抗体的水平在 DNA 免疫组是比那低的。同时， anti-SSB 抗体的水平比在合作免疫组的 anti-DNA 抗体的高。不词法或免疫学的畸形在心，肝，肾，脾或皮被发现纸巾。结论：anti-dsDNA-antibody 的抑制被合作免疫与综合 SSB 肽和 DNA 导致，它可能解释，至少部分，在与 SSB 联系的一些 LE 子集的温和疾病抗体。

英文摘要：

Background Patients with systemic lupus erythematosus often have various autoantibodies. The relationship between these antibodies is still poorly understood. The aim of the present study was to observe the anti-SSB antibody and anti-dsDNA antibody production profiles following immunization with synthetic SSB peptide alone, DNA alone or co-immunization with these two antigens. Methods SSB 214-225 aa peptide was synthesized by organic chemistry solid-phase peptide synthesis. Rabbits were immunized with the following antigens： synthetic SSB peptide linked with keyhole limpet hemocyanin （KLH）, DNA, SSB plus dsDNA, KLH and PBS. Antibodies were measured by ELISA. Histopathology and direct immufluorescence assays were also applied. Results Anti-SSB and anti-dsDNA antibodies were produced following immunization with SSB peptide and DNA respectively. The level of SSB antibody in the co-immunization group was higher than that of the SSB peptide immunization group. The level of anti-dsDNA antibody in the co-immunization group was, however, lower than that in the DNA immunization group. Meanwhile, the level of anti-SSB antibody was higher than that of anti-DNA antibody in the co-immunization group. No morphological or immunological abnormalities were found in the heart, liver, kidney, spleen or skin tissues. Conclusion Inhibition of anti-dsDNA-antibody was induced by co-immunization with synthesized SSB peptide and DNA, which might explain, at least partly, the mild disease in some LE subsets associated with SSB antibody.