中文摘要：

目的：研究左、右归丸及其拆方对去卵巢大鼠肝脏LPL、PPAR-γ基因与蛋白表达的影响,从而在分子水平说明其影响脂质代谢的机制。方法：90只2月龄SD大鼠,随机分为正常组（Control）,假手术组（SHAM）,模型组（OVX）,补佳乐组（BJL）,左归丸组（ZGW）,右归丸组（YGW）,共同药组（GTY）,滋阴药组（ZYY）,补阳药组（BYY）,每组10只,正常组不做处理,假手术组麻醉后开腹切除卵巢周围适量脂肪组织,其余各组常规切除双侧卵巢。手术后1周,分别以蒸馏水、蒸馏水、蒸馏水、阳性对照药戊酸雌二醇片混悬液、左归丸、右归丸、两方共同药、滋阴药、补阳药的水煎液给各组大鼠灌胃。12周后处死大鼠,取大鼠肝脏,用实时荧光定量PCR技术检测脂蛋白酯酶（LPL）、过氧化物酶体增殖物激活受体-γ（PPAR-γ）的mRNA表达,用免疫组化方法检测大鼠肝脏LPL、PPAR-γ的蛋白表达。结果：与模型组比较,左、右归丸及其拆方均能上调LPL、PPAR-γmRNA的表达水平（P〈0.01）,上调LPL、PPAR-γ蛋白的表达水平。结论：左、右归丸及其拆方均可上调肝脏中LPL、PPAR-γ基因与蛋白的表达水平,这可能是其调节脂质代谢的机制之一。

英文摘要：

Objective：To study the influence of Zuogui Pill, Yougui Pill and demolition parts on the gene and protein expression of LPL and PPAR - γ in ovariectomized rats livers, and thus explaining the mechanism of the influence on lipid metabolism at the molecular level. Methods ： A total of 90 two - month - old female SD rats were divided into nine groups ： normal control group （ Control）, sham operated group（ SHAM ）, ovariectomized group （ OVX ） Zuogui Pill group （ ZGW）, Yougui Pill group （ YGW）, mutual drugs of Zuogui Pill and Yougui Pill group（ GTY）, nourishing kidney- Yin drugs group（ ZYY）, tonifying kidney -Yang drugs group（BYY） ,and there were 10 rats in each group. The Control did not make any processing. The SHAM took the appropriate adipose tissue by open surgery after anesthetization and the rest of groups were routinely removed ovary. After 1 week, the SD rats were gavaged with distilled water, distilled water, distilled water and the positive control drug （Estradiol Valerate, BJL） sus- pension,the water decoction of Zuogui Pill, Yougui Pill, mutual drugs of Zuogui Pill and Yougui Pill, nourishing kidney - Yin drugs,tonifying kidney -Yang drugs. Give medicine for 12 weeks, the SD rats were killed. Take the fresh livers and then detect the Lipoprotein lipase （LPL） and perxisome proliferators - activate receptor - γ （ PPAR - γ） mRNA by the method of fluores- cence quantitative PCR （ Q - PCR）. Detect the protein expressions of LPL and PPAR - γ by the method of immunohistochemis- try. Results ： Compared with the model group, Zuogui Pill and Yougui Pill and their demolition parts all can significantly up - regu- late the expressions of LPL and PPAR - γ mRNA（ P 〈 0. 01 ） and can significantly rise the positive expressions of LPL and PPAR -3＇ immunohistochemistry. Conclusion：Zuogui Pill;Yougui Pill and their demolition parts all can improve the gene and protein expressions of LPL and PPAR - γ in liver. This probably is one of the mechanism of reg