中文摘要：

Mitochondrial 细胞色素 b (Cytb ) ，很少蛋白质之一由 mitochondrial DNA 编码了，在转移电子起一个重要作用。作为 mitochondrial 基因，它广泛地为种系发生的分析被使用了。以前，编辑的编码基因和 mRNA 的 949-bp 碎片从 Prorocentrum donghaiense 被描绘，它可能为解决 P 证明有用。从密切相关的种类的 donghaiense。然而，全身的编码区域没被描绘。在这研究，我们使用了 cDNA 结束(种族) 的快速的扩大获得全身， 1 124 bp cDNA。Cytb 抄本包含了标准开始 codon ATG，但是没有可认识的站 codon。相同比较出现了 P。donghaiense Cytb 从另外的 dinoflagellate 种类有高顺序身份到 Cytb 序列。种系发生的分析把 Cytb 放从 P。在从 P 与那强烈一起聚类的 dinoflagellates 和它的 clade 的 donghaiense。最小。基于全身的顺序，我们推断了 32 在不同位置编辑事件，为 2.93% Cytb 基因的财务。( 11 )34.4%变化是到 G 的 A ，( 8 )25%是到 C 的 T ，并且( 8 )25%是到 U 的 C ，与到 C 的 G 和到 A 的 G 的更小的比例编辑(9.4%( 3 )并且6.2%( 2 )，分别地)。Cytb 抄本的表示水平被即时 PCR 在整个生长阶段期间在不同时间与一根 TaqMan 探针确定。平均 Cytb 抄本在 39.27 慢慳瑬琠慨 ? 桴瑡椠 ? 牧湡瑩 ?敢慣獵 是在场的吗？？

英文摘要：

Mitochondrial cytochrome b （Cytb）, one of the few proteins encoded by the mitochondrial DNA, plays an important role in transferring electrons. As a mitochondrial gene, it has been widely used for phylogenetic analysis. Previously, a 949-bp fragment of the coding gene and mRNA editing were characterized from Prorocentrum donghaiense, which might prove useful for resolving P. donghaiense from closely related species. However, the full-length coding region has not been characterized. Ih this study, we used rapid amplification of cDNA ends （RACE） to obtain full-length, 1 124 bp cDNA. Cytb transcript contained a standard initiation codon ATG, but did not have a recognizable stop codon. Homology comparison showed that the P. donghaiense Cytb had a high sequence identity to Cytb sequences from other dinoflagellate species. Phylogenetic analysis placed Cytb from P. donghaiense in the clade of dinoflagellates and it clustered together strongly with that from P. minimum. Based on the full-length sequence, we inferred 32 editing events at different positions, accounting for 2.93% of the Cytb gene. 34.4% （11） of the changes were A to G, 25% （8） were T to C, and 25% （8） were C to U, with smaller proportions of G to C and G to A edits （9.4% （3） and 6.2% （2）, respectively）. The expression level of the Cytb transcript was quantified by real-time PCR with a TaqMan probe at different times during the whole growth phase. The average Cytb transcript was present at 39.277.46 copies of cDNA per cell during the whole growth cycle, and the expression of Cytb was relatively stable over the different phases. These results deepen our understanding of the structure and characteristics of Cytb in P. donghaiense, and confirmed that Cytb in P. donghaiense is a candidate reference gene for studying the expression of other genes.