中文摘要：

NF-κB是参与免疫调节、炎症反应、肿瘤发生等过程重要的转录因子,在TNFα、IL-1β、LPS等因子诱导下NF-κB发生核转位并激活其转录活性。尝试利用高内涵筛选（High Content Screening,HCS）和RNA干扰（RNAinterference,RNAi）技术建立NF-κB核转位高通量筛选体系,该体系的应用将有助于发现新的NF-κB信号通路调节因子,为免疫与肿瘤的机制研究提供线索。

英文摘要：

NF-κB is an important transcription factor that regulate immune reaction,inflammation,tumorigenesis,etc.In response to some cytokines,such as TNFα,IL-1β and LPS,NF-κB translocates from cytoplasm to nuclear and its transcriptional activity is activated.Using high content screening（HCS） and RNA interference（RNAi） technology build a NF-κB high-throughput screening system through quantifing nuclear translocation signals.The application of this screening system will help us to discover the new regulator of NF-κB signaling pathway and reveal the mechanism of immunity and tumorigenesis.