中文摘要：

目的：分析γ-亚麻酸3种不同作用途径对小鼠血清中24种细胞因子的影响,进而探索γ-亚麻酸对小鼠免疫系统的调节作用。方法：将γ-亚麻酸以口服灌胃、腹腔注射、耳静脉注射3种方式作用于小鼠,分别在3、2、2 h后眼球取血,分离血清。采用液体芯片检测技术测定小鼠血清中2 4种细胞因子含量的变化。结果：口服灌胃组中,G-CSF、MCP-1、MIP-1α、VEGF、IL-1α、IL-3、IL-10、IL-13,8种细胞因子显著变化;腹腔注射组中有IFN-γ、TNF-α、IL-1β、IL-5、IL-6、IL-10,6种细胞因子显著变化;耳静脉注射组中G-CSF、KC、IP-10、IL-1α、IL-4、IL-12（p40）、IL-17 7种细胞因子显著变化。根据每种作用途径显著变化的细胞因子的产生及作用靶细胞,分别构建γ-亚麻酸作用后机体细胞通讯网络的变化。结论：γ-亚麻酸能通过小鼠胃肠黏膜系统和血液系统传递信号,调节机体的免疫,其作用的信号途径涉及NF-κB、P38/JNK/MAPK、JAK-STAT等途径。γ-亚麻酸不仅具有必需脂肪酸的作用,而且具有显著的免疫调节作用,不仅具有刺激炎症作用,同时还可以发挥抗炎症作用。

英文摘要：

Objective： To analyze the effects of three different administration approaches of γ-linolenic acid on 24 cytokines in mouse serum,with the purpose to reveal the immunoregulatory effect of γ-linolenic acid Methods： γ-linolenic acid was given to mice by intragastric administration,intraperitoneal injection or ear vein injection and serum was collected from eye ball at 3,2 and 2 h after administration of γ-linolenic acid,respectively.Luminex xMAP technology was used to determine 24 cytokines in mouse serum.Results： 8 cytokines,6 cytokines,and 7 cytokines,respectively,showed significant changes after intragastric administration,intraperitoneal injection and vein injection of γ-linolenic acid.Based on the change patterns of cytokines after administration of γ-linolenic acid,cells that secrete these cytokines and their target cells were analyzed and intercellular communication networks responding to these administration methods were proposed.Conclusion： γ-Linolenic acid signals through mouse gastrointestinal mucosa and blood system to regulate the immune system.Signal transduction might involve NF-KB,P38/JNK/MAPK,JAK-STAT etc.γ-Linolenic acid not only is an essential fatty acid,but also has significant immunoregulatory effects.It stimulates inflammation and plays an anti-inflammatory role as well.