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中文摘要：
研究了不同浓度表面活性剂Tween-80，Triton X-100，SDS对大肠杆菌生产α-环糊精葡萄糖基转移酶（α-CGT酶）的影响。结果表明：发酵初始添加Tween-80和TritonX-100的最适浓度分别为2％，0．5％，最终胞外酶活分别达2．03U／ml和4．92U／ml，相对于未添加表面活性剂时提高4．6倍和12．67倍，且改变添加时间不能提高酶的产量；发酵36h添加0．02％SDS对α-CGT酶产量促进最大，最终胞外酶活达5．31U／ml，较对照组提高12．75倍。表面活性剂对α-CGT酶生产的促进作用可能是由大肠杆菌细胞内外膜渗透性增加所致，使细胞周质空间中α-CGT酶能更加快速地渗透到胞外。
英文摘要：
The effect of surfactants on the production of recombinant extracellular α-CGTase in E. coli was studied. The result indicated that the optimum concentration of Tween-80 and Triton X-100 are 2% and 0.5% when addition time of surfactant was performed at the beginning of culture. In such condition, α-CGTase activity of culture supernatant reached 2.03 U/ml for Tween-80 and 4.92 U/ml for Triton X-100 and were respectively 4.6 and 12.67 higher than that without addition of surfactant. The different addition time during culture stage could not further improve the yield. When adding 0.02% SDS at 20 h culture, the final yield of enzyme could reach to 5.31 U/ml, which was 12.75 times higher than that of control. The enhanced extracellular secretion of α-CGTase by surfactants is possibly caused by the increasing of the cell membrane pemeabilization of E. coli.
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