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中文摘要：
为分析从某鹅病料样品中分离鉴定的一株新城疫病毒（NDV）（Goose／Foshan／2010）F和HN基因的序列特征，本研究采用RT．PCR方法扩增该病毒的F和HN基因的ORF序列，并进行序列测定。经序列比对、进化分析表明，Goose／Foshan／2010株属于基因VIIb亚型病毒株，F蛋白裂解位点序列为^112RRQKRF^117，HN蛋白由571个氨基酸残基组成，具有强毒株分子特征。对F和HN蛋白分析表明，其HN蛋白存在E347D和K495E两个点突变；此外，HN蛋白缺失了538位-540位的糖基化位点。该分离株为我国首次报道的鹅源基因Ⅶb亚型NDV分离株，与秘鲁及马来西亚早期基因Ⅶb亚型分离株进化关系密切，表明我国目前新城疫的流行情况十分复杂。
英文摘要：
In this study, a Newcastle disease virus （NDV） was isolated from goose in Sanshui district, Foshan, designated Goose/Foshan/2010. The ORF sequences of F and HN gene of the isolate were amplified by RT-PCR and sequenced. The aligning analysis results showed that the isolate was placed into genotype Ⅶb of NDV with a F protein cleavage motif of 112R-RQKR-F117 which was the molecular character of the virulent NDV. Two point mutations of E347D and K495E occurred in neutralizing epitopes of in HN protein. Furthermore, a glycosylation site was deleted in HN protein at aa 538-aa 540. Phylogenetic analysis showed that the Goose/Foshan/2010 was closely related to the early isolates of genotype Ⅶb from Peru and Malaysia. While, it is the first report of genotype Ⅶb NDV isolated from goose in China.
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