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中文摘要：
分别利用磁镊和原子力显微镜研究阿司匹林与不同类型双链DNA分子的相互作用。在2×10^-4mol/L浓度的阿司匹林作用下,在磁镊的单分子力谱中观察到DNA的B-A构象的转变,且其持久长度由52 nm减小到25 nm左右;利用原子力显微镜观察阿司匹林对线状DNA的弯折和凝聚作用,发现其程度随阿司匹林浓度的增加而增强;而对环状DNA,阿司匹林可使其发生缠绕,且随着阿司匹林浓度的增加,单个DNA分子上面的结点数也会增加,最后缠绕成棒状结构或者球状。同时,利用动态光散射技术,进一步测量了阿司匹林分子导致的λ-DNA粒径的变化,药物浓度由2×10^-6mol/L增加到2×10-4mol/L,DNA的粒径由135 nm逐渐减小到56 nm左右。根据实验结果,建立了阿斯匹林导致DNA构象变化的唯象模型。
英文摘要：
The interaction between aspirin and double-stranded DNA was studied by using magnetic tweezers and atomic force microscope, respectively. When the concentration of aspirin was 2×10^-4 mol/L, the B-A conformation transition of the DNA was observed via single molecular force spectrum of magnetic tweezers, and results showed the persistent length decreased from 52 to 25 nm. The folding and condensation of DNA induced by aspirin was observed using AFM, it was found that the binding number of single circle DNA molecules and aspirin increased with aspirin concentration increasing, and finally condensed into the morphologies of bar or globular. Meanwhile, the authors measured the particle size of complex after the interaction of DNA and aspirin and found the particle size decreased from 135 to 56 nm when the aspirin concentration increased from 2×10^-6 to 2×10^-4 mol/L. According to experimental results, the phenomenological model of DNA conformation change induced by aspirin was built.
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