中文摘要：

目的研究胚胎心肌细胞兴奋收缩耦联的机理；方法使用膜片钳与钙离子浓度分析系统测量酶消化法得到的小鼠胚胎心肌细胞的膜电位与细胞内钙离子浓度；结果细胚胎心肌细胞存在两种兴奋收缩耦联模式，一种与正常成熟心肌细胞的兴奋收缩耦联模式类似，与细胞膜上的钠通道、L-钙离子通道相关；另一种由细胞内钙振荡诱发，这种钙振荡通过细胞膜上的钠钙交换蛋白引起了细胞膜电位的小幅度变化，该模式是一种更基本的兴奋收缩模式。近似熵分析表明，与后一种模式相比较，前一种模式的规律性更强。结论胚胎心肌细胞存在两种兴奋收缩耦联模式。

英文摘要：

Objective To find the mechanism for excitation-contraction （E-C） coupling modes in embryonic cardio- myocytes; Method The membrane potential and intracallular Calcium were respectively measured with whole call patch clamp and Calcium imaging methods; Results There exist two kinds of E-C coupling modes. One is similar to the E-C coupling mode of mature cardio-myocytas and its membrane potential was related to Sodium Channel and L-Calcium channel. The other mode is more basic for embryonic cardio-myocytes, which is controlled by intracallular Calcium oscillationto cause the slight change of membrane potential through Na＋-Ca2＋ exchanger. Compared to the second one, the first mode is more regular. Conclusion Two kinds of E-C coupling modes exist for embryonic cardiomyocytes.