中文摘要：

背景：研究表明，钛金属纳米管涂层不仅能够促进材料本身的生物活性，同时还可作为药物载体负载抗生素及生长因子等。目的：检测万古霉素/羟基磷灰石/二氧化钛纳米管的体内外抗菌性能。方法：检测万古霉素/羟基磷灰石/二氧化钛纳米管、万古霉素/二氧化钛纳米管的体外释药性能。将1010 L-1的金黄色葡萄球菌稀释液分别铺于商业钛金属、二氧化钛纳米管及万古霉素/羟基磷灰石/二氧化钛纳米管上，24 h 后，扫描电镜观察细菌生长情况，聚焦扫描电镜观察细菌活性。结果与结论：①扫描电镜观察：金黄色葡萄球菌在万古霉素/羟基磷灰石/二氧化钛纳米管上的数量最少且细菌形态破坏；②聚焦扫描电镜观察：万古霉素/羟基磷灰石/二氧化钛纳米管上的细菌数量及活菌数量最少，商业钛金属上的活菌数量最多；③体外释药性能：万古霉素从羟基磷灰石/二氧化钛纳米管上的释放时间长达18 d，从二氧化钛纳米管上的时间仅仅约为4 h；④结果表明：万古霉素/羟基磷灰石/二氧化钛纳米管具有良好的抗菌性能及缓释性能。

英文摘要：

BACKGROUND： Present studies have proved that titanium coating nanotubes cannot only promote the biological activity of the material itself, but also be used as a drug carrier loading antibiotics and growth factors. OBJECTIVE： To investigate the antibacterial properties of vancomycin/hydroxyapatite/titanium dioxide nanotubes in vitro and in vivo. METHODS： The releasing property in vitro of vancomycin/hydroxyapatite/titanium dioxide nanotubes and vancomycin/titanium dioxide nanotubes were detected. And 1010/L Staphylococcus aureus dilution was put onto the commercial titanium, titanium dioxide nanotube and vancomycin/hydroxyapatite/titanium dioxide nanotube, respectively. Twenty-four hours later, the bacterial growth and activity was observed by scanning electron microscope and confocus scanning electron microscope, respectively. RESULTS AND CONCLUSION： Scanning electron microscope showed： the number of Staphylococcus aureus was the least on the vancomycin/hydroxyapatite/titanium dioxide nanotube, and the bacterial morphology was destroyed. Confocus scanning electron microscope observed： the number of bacteria and viable bacteria was the least on the vancomycin/hydroxyapatite/titanium dioxide nanotube, and the most on the commercial titanium. Besides, the releasing time of vancomycin from the hydroxyapatite/titaniumdioxide nanotube was up to 18 days, but the releasing time of vancomycin was only 4 hours from the titanium dioxide nanotube. In conclusion, the vancomycin/hydroxyapatite/titanium dioxide nanotube has good antibacterial property and slow-releasing performance.