中文摘要：

目的建立高效液相色谱分析法测定性早熟患儿血清中4种环境内分泌干扰物（DEHP、MEHP、p,p＇-DDE、4-NP）含量的方法。方法正己烷提取血清样品,采用C18色谱柱分离。以甲醇：水：三氟乙酸（1000：50：1）为流动相,应用紫外检测器在波长228nm处检测DEHP和MEHP,在波长238nm处检测p＇-DDE；应用荧光检测器在激发波长275nm、发射波长312nm处检测4-NP。结果血清中待测组份的浓度和峰面积呈良好的线性关系,的线性范围是0.002～1.0μg/m。检测限均为1ng/ml。回收率DEHP为96.84%～103.44%,MEHP为87.69%～104.92%,p,p＇-DDE为87.6%～109.89%,4-NP为98.57%～105.1%。结论该方法样品处理简单、操作简便,能够节省生物样品,缩短检测时间,提高检测效率。

英文摘要：

Objective This study was directed to the development of a simple and highly sensitive method for determination of four kinds of environmental endocrine discrupters（EEDs） in the serum of precocious puberty patients using reversed-phase HPLC. Methods The serum samples were extracted by n-hexane. The operating conditions were such as C18 column（4.6mm×250μm 5μm）, methanol： H2 O： trifuorocuetic acid（ 1000：50：1 ）as mobile phase at a flow rate of 1ml/min. Using ultraviolet detector, DEHP and MEHP was determined with wavelength at 228nm, p, p＇-DDE was determined with wavelength at 238nm. Fluorescence detector with the excitation and emission wavelength at 275nm and 312nm respectively were used to determine 4-NP. Results There was a good linear relationship between the concentrations of the analytes in serum and their peak areas in the range of for DEHP, for MEHP, for p, p＇-DDE and for 4-NP. The detection limit of the method was 1ng/ml for each of the analytes. Recoveries of serum samples were 96.84% - 103.44% for DEHP, 87.69%- 104.92% for MEHP,87.6%- 109.89%for p,p＇-DDE,98.57%- 105.1% for 4-NP. Conclusion The method is suitable for the determination of the four kinds of EEDs in serum. It is easy to dispose the samples. The volume of samples is decreased and the effect of detection is increased. The method is quicker and easier and could be widely used to determine these compounds. It is helpful for the clinical study of precocious puberty.