中文摘要：

目的监测和分析2011年和2013年武汉市婴幼儿和成人病毒性胃肠炎病原。方法用聚丙烯酰胺凝胶电泳及RT-PCR对胃肠炎患者腹泻大便样本进行轮状病毒、诺如病毒、星状病毒以及札幌样病毒检测,部分样本测序并比对同源性。用PCR检测腺病毒。结果轮状病毒、诺如病毒、星状病毒、札幌样病毒检出率分别为21.6%（296/1 368）、35.7%（488/1 368）、10.2%（140/1 368）和7.7%（105/1 368）;肠道腺病毒在儿童患者中的检出率为8.2%（70/853）。诺如病毒和轮状病毒易感人群为13～24月龄和7～24月龄儿童,及40～59岁成人。轮状病毒检出高峰在秋冬季。星状病毒、札幌样病毒和腺病毒多发于2岁以下的婴幼儿,无明显季节分布。196人份A组轮状病毒基因型以G9P[8]为主（40.8%）,其次为G1P[8]（26.5%）,G3P[8]（16.3%）和G2P[4]（11.7%）。经测序比对诺如病毒基因型分别为GⅡ.4型（7/12）,GⅡ.3型（5/12）;星状病毒为1型（8/13）,8型（4/13）和5型（1/13）;札幌样病毒为GI.1型（2/6）,GI 2型（2/6）和GⅡ.1型（2/6）。结论 2011年和2013年武汉市婴幼儿和成人胃肠炎主要病原是诺如病毒,其次是轮状病毒。

英文摘要：

To survey and analyze the pathogens of viral gastroenteritis among infants and adults in 2011 and 2013in Wuhan,the stool samples were detected for the rotavirus,norovirus,astrovirus and sapovirus by polyacrylamide gel electrophoresis（PAGE）,RT-PCR and PCR.The nucleotide sequences of partial positive samples were determined and their identities were analyzed by BLAST and/or genotyping tool online.The detection rates of rotavirus,norovirus,astrovirus,sapovirus were21.6%（296/1 368）,35.7%（488/1 368）,10.2%（140/1 368）and 7.7%（105/1 368）,respectively.The detection rate of adenovirus in children was 8.2%（70/853）.The detection rates of norovirus and rotavirus were higher in 13-24 months and 7-24 months age groups,respectively,and 40-59 years old age group.The detection rate of rotavirus was higher in autumn and winter.Astrovirus,sapovirus and adenovirus were more frequently detected in children under 2years old.Seasonality was not observed in astrovirus,sapovirus or adenovirus.The genotypes of 196 group A rotavirus were determined.Of these,the most frequent were G9P[8]（40.82%）,followeing by G1P[8]（26.53%）,G3P[8]（16.33%）and G2P[4]（11.73%）.The genotypes of norovirus were GII 4（7/12）and GII 3（5/12）,and that of astrovirus were type 1（8/13）,type 8（4/13）and type 5（1/13）.The genotypes of sapovirus were GI.1（2/6）,GI.2（2/6）and GII.1（2/6）.The main pathogens of viral gastroenteritis were norovirus and rotavirus in 2011 and 2013,in Wuhan.