中文摘要：

引言 烃降解微生物接触烃类有3种方式[1]：一是主动接近并附着在大分子烃类物质上,利用细胞膜上的烷烃加氧酶改变烃类性质;二是产生表面活性剂类物质将石油烃乳化成小液滴,进而靠主动运输摄取交换;三是直接将大分子烃类物质运输至胞内利用。无论以哪种方式,

英文摘要：

In this report,the chemotaxis properties of an oil-degrading strain 6-1B using the swarm plate assay and the characteristics of chemotaxis movement towards crude oil are described.The strain 6-1B,isolated from formation water samples from Daqing Oilfield,China,was determined as Pseudomonas sp.（99.9% identity）by 16S rDNA sequence analysis and physiological and biochemical characteristics.The organism was shown to utilize crude oil as the sole carbon and energy source.Cell growth and crude oil degradation occurred at 42℃.As many natural isolates of Pseudomonas sp.,strain 6-1B was highly motile with a polar flagella and was found to be chemotactic towards a variety of metabolites including glycine,glycerol and sucrose.Moreover,chemotaxis was also observed towards crude oil and n-alkenes such as tridecane.The maximum chemotaxis rate was 35 μm·s-1,while the maximum bacterial concentration occurred in the oil-water interface.