中文摘要：

目的 应用抗体芯片技术检测慢性肾脏病（CKD）患者尿液中细胞因子的水平，并探讨其临床意义。方法 研究对象共10例，7例为CKD患者，全部符合K／DOQI指南中CKD的诊断标准，并且有肾脏病理诊断。依据GFR水平以及CKD分期，将7例患者分为两组：Ⅰ组：GFR≥80ml·min^-1·（1．73m^2）^-1（CKD1～2期）共4例；Ⅱ组：GFR≤40ml·min^-1·（1．73m^-2）^-4（CKD3～5期）共3例。另选取3例性别、年龄相匹配的健康人作为正常对照。应用Raybiotech人类细胞因子抗体芯片，同时检测各组尿液中20种细胞因子水平的变化。结果 与正常对照组相比，CKD患者共有15种因子的水平发生了显著变化。单核细胞趋化蛋白（MCP）-1、RENTES、金属蛋白酶组织抑制物（TIMP）-1、肿瘤坏死因子（TNF）-α、血管内皮生长因子（VEGF）、E-选择素（seletin）、Fas、细胞间黏附分子（ICAM）-1、白细胞介素（IL）-2、基质金属蛋白酶（MMP）-2、转化生长因子（TGF）-β和血小板源生长因子（PDGF）-BB的水平同正常对照组相比升高了2～5倍，其中尿MCP-1，RANTES，TIMP-1，TNF-α和VEGF的水平随着GFR的下降而进一步升高。VCAM-1和PDGF-BB的水平同正常对照组相比有所下降。在芯片所包含的20种细胞因子中，MMP-9的水平变化尤其显著，同正常对照组相比，CKDI组是正常对照组的492．8倍，CKDⅡ组是正常对照组的198．7倍。结论 首次应用抗体芯片技术，证实CKD患者尿液中细胞因子表达水平同正常对照组有明显差异，并且与疾病所处阶段有一定的关系。

英文摘要：

Objective To apply a novel proteomic technology to analyze urinary cytokine profiles in patients with CKD. Methods Ten subjects including 7 CKD patients and 3 normal controls were enrolled in this study. Differential excretion of urinary cytokines was determined by human cytokines antibody array （Raybiotech Norcross GA）. As compared to controls, a two-fold change in spot intensity was considered significant. Results A total of 15 cytokines varied significantly in urinary samples from patients with CKD, comparing with controls. It was shown that MCP-1, R-ANTES, TIMP- 1,TNF-α, VEGF, E-selectin, Fas, ICAM-1, IL-2, MMP-2, TGF-β increased by two to five folds in CKD patients with normal renal function, while the excretion of MCP-1, RANTES, TIMP-1, TNF-α and VEGF was further increased with the declining of renal function. A decreasing excretion of urinary VCAM-1 and PDGF was found in patients with renal failure. Impressively, the urinary MMP-9 excretion was 492 folds increase in CKD patients with normal renal function and 198 folds increase in renal failure patients as compared to normal controls. Conclusion A significant change of urinary cytokine profiles in patients with CKD is firstly demonstrated as compared to normal controls by using a novel antibody array technology, which may provide important information regarding to the role of cytokines in the progression of CKD.